EVALUATION OF THE EFFECT OF FEEDING ON PORTULACA OLERACEA SEED OIL ON BIOSYNTHESIS OF LONG CHAIN POLYUNSATURATED FATTY ACIDS IN RATS.
- Biochemistry Department, Faculty of Agriculture, Menoufia University.
- Abstract
- Keywords
- References
- Cite This Article as
- Corresponding Author
Feeding on foods with high levels of omega 3 (n-3) fatty acids is a major challenge in Egypt because most of the oils used in nutrition are characterized by high content of n-6 fatty acids and low content of n-3 fatty acids. It is so difficult to obtain n-3 fatty acids from cold-water fish for their high price and mercury pollution. So you should look for new alternatives such as purslane (Portulaca oleracea L.) seed oil which is rich in α-linolenic acid (ALA), the precursor of the rest of the omega 3 fatty acids (n-3) family (C20:5 or eicosapentaenoic acid [EPA] and C22:6 or docosahexaenoic acid [DHA]). This study is designed to evaluate the hepatic bioconversion of ALA and linoleic (LA) to long chain polyunsaturated fatty acids (LCPUFA) and the statue of liver in male albino rats fed on purslane seed oil. Three experimental groups (n= 10 rats each group) were feed on these three different oils (sunflower oil, olive oil and purslane seed oil) for 30 days. Purslane seed oil increased ALA, EPA and DHA in liver compared with sunflower oil and olive oil groups without any changes in the parameters which express liver damage. It is concluded that purslane seed oil may be good nutritional source for providing n-3 fatty acid (ALA, EPA and DHA).
- Abdalla, H.M. (2010): Purslane extract effects on obesity-induced diabetic rats fed a high-fat diet. Malays. J. Nutr., 16: 419-429.
- Abdalla, H.M. and Mohamed, S.A.G. (2010): In vivo hepato-protective properties of purslane extracts on paracetamol-induced liver damage. Malays. J. Nutr., 16: 161-170.
- Abete, P., Testa, G., Galizia, G., Della-Morte, D., Cacciatore, F. and Rengo, F. (2009): PUFA for human health: diet or supplementation? Curr. Pharm. Des., 15: 4186-4190.
- Alsted, A. and Hoy, C. (1992): Fatty acid profiles of brain phospholipid subclasses of rats fed n-3 polyunsaturated fatty acids of marine or vegetable origin, a two generation study. Biochim. Biophys. Acta, 1125: 237?244.
- Araya, J., Rodrigo, R., Videla, L.A., Thielemann, L., Orellana, M., Pettinelli, P. and Poniachik, J. (2004): Increase in longchain polyunsaturated fatty acid n6/n 3 ratio in relation to hepatic steatosis in patients with non- alcoholic fatty liver disease. Clin. Sci., 106: 635-643.
- Araya, J., Rodrigo, R., Pettinelli, P., Araya, A.V., Poniachik, J. and Videla, L.A. (2010): Decreased liver fatty acid D-6 and D-5 desaturase activity in obese patients. Obesity, 18: 1460-1463.
- Bancroft, J.D., Stevens, A. and Turner, D.R. (1996): Theory and practice of histological techniques. 4th ed Churchill Livingstone; Edinburgh, London, Melbourne.
- Bell, J.G., McGhee, F., James, R. and Dick Douglas, R.T. (2005): Dioxin and dioxin-like polychlorinated biphenyls (PCBs) in Scottish farmed salmon (Salmo salar): effects of replacement of dietary marine fish oil with vegetable oils. Aquacult., 243: 305-314.
- Bligh, E.G. and Dyer, W.J. (1959): A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37: 911-917.
- Brenner, R.R. (2003): Hormonal modulation of D6 and D5 desaturases: case of diabetes. Prostag. Leukotr. Ess., 68: 151-162.
- Cho, H.P., Nakamura, M. and Clarke, S.D. (1999): Cloning, expression, and fatty acid regulation of the human delta-5 desaturase. J. Biol. Chem., 274: 37335-9.
- Cunnane, S.C. (2003): Problems with essential fatty acids: time for a new paradigm? Prog. Lipid Res., 42: 544-568.
- Dinh, T.K.L., Bourre, J.M., and Durand, G. (1993): Effect of Age and α-Linolenic Acid Deficiency on Δ6 Desaturase Activity and Liver Lipids in Rats, Lipids, 28: 517?523.
- Ebrahimzadeh, M.A., Nabavi, S.F. and Nabavi, S.M. (2009): Essential oil composition and antioxidant activity of Pterocarya fraxinifolia. Pak. J. Biol. Sci., 12: 957-963.
- Emken, E.A., Adlof, R.O. and Gulley, R.M. (1994): Dietary linoleic acid influences desaturation and elongation of deuterium labelled linoleic and linolenic acids in young adult males. Biochim. Biophys. Acta, 1213: 277?288.
- Fetterman, J.W. and Zdanowicz, M.M. (2009): Therapeutic potential of n-3 polyunsaturated fatty acids in disease. Am. J. Health Syst. Pharm., 66: 1169-1179.
- Firestone, D. (1997): In Official Methods of Analysis of AOAC International (Cunniff, P., Ed.), pp. 963-964, Assoc. Official Anal. Chem., Gaithersburg, MD.
- Harris, W.S. (2009): The omega-3 index: from biomarker to risk marker to risk factor. Curr. Atheroscler. Rep., 11: 411-417.
- Holman, R.T. (1998): The slow discovery of the importance of omega 3 essential fatty acids in human health. J. Nutr., 128: 427S-433S.
- Kamal-Eldin, A. and Andersson, R. (1997): A multivariate study of the correlation between tocopherol content and fatty acid composition in vegetable oils. J. Am. Oil Chem. Soc., 74: 375-380.
- Kanai, S., Uto, K., Honda, K., Hagiwara, N. and Oda, H. (2011): Eicosapentaenoic acid reduce warfarin-induced arterial calcification in rats. Atherosclerosis, 215: 43-51.
- Lands, W.E., Libelt, B., Morris, A., Kramer, N.C., Prewitt, T.E., Bowen, P., Schmeisser, D., Davidson, M.H. and Burns, J.H. (1992): Maintenance of Lower Proportions of (n-6) Eicosanoid Precursors in Phospholipids of Human Plasma in Response to Added Dietary (n-3) Fatty Acids, Biochim. Biophys. Acta, 1180: 147?162.
- Li, D. and Hu, X. (2009): Fish and its multiple human health effects in times of threat to sustainability and affordability: are there alternatives? Asia Pac. J. Clin. Nutr., 18: 553-563.
- Liang, X., Tian, J., Li, L., Gao, J. and Zhang, Q. (2014): Rapid determination of eight bioactive alkaloids in Portulaca oleracea L. by the optimal microwave extraction combined with positive-negative conversion multiple reaction monitor (+/-MRM) technology. Talanta, 120: 167-172.
- Liu, L., Howe, P., Zhou, Y.F., Xu, Z.Q. and Hocart, C. (2000): Fatty acids and betacarotene in australian purslane (Portulaca oleracea) varieties. J. Chromatogr. A, 893: 207-213.
- Liu, L., Xie, C., Yue, L., Jing, S. and Cai Y. (2014): Preparation of Portulaca oleracea L. seed oil by ultrasound-assisted enzyme hydrolysis combined with Soxhlet extraction method and the analysis of its fatty acids. Food Ferment. Ind., 40: 218-222.
- Mandal, C.C., Ghosh-Choudhury, T., Yoneda, T., Choudhury, G.G. and Ghosh-Choudhury, N. (2010): Fish oil prevents breast cancer cell metastasis to bone. Biochem. Biophys. Res. Commun., 402: 602-607.
- Molendi-Coste, O., Legry, V. and Leclercq, I.A. (2011): Why and How Meet n-3 PUFA Dietary Recommendations? Gastroenterol. Res. Pract., Article ID 364040.
- Nakamura, M.T. and Nara, T.Y. (2004): Structure, function, and dietary regulation of D6, D5, and D9 desaturases. Annu. Rev. Nutr., 24: 345-376.
- Oliver, E., McGillicuddy, F., Phillips, C., Toomey, S. and Roche, H.M. (2010): The role of inflammation and macrophage accumulation in the development of obesity-induced type 2 diabetes mellitus and the possible therapeutic effects of long-chain n-3 PUFA. Proc. Nutr. Soc., 69: 232-243.
- Orellana, M., Varela, N., Guajardo, V., Araya, J. and Rodrigo, R. (2002): Modulation of rat liver cytochrome P450 activity by prolonged red wine consumption. Comp. Biochem. Physiol. C. Toxicol. Pharmacol., 131: 161-166.
- Osman, S.M. and Hussein, M.A. (2015): Purslane seeds fixed oil as a functional food in treatment of obesity induced by high fat diet in obese diabetic mice. J. Nutr. Food Sci., 5: 332. doi:10.4172/2155-9600.1000332.
- Picq, M., Chen, P., Perez, M., Michaud, M., Vericel, E., Guichardant, M. and Lagarde, M. (2010): DHA metabolism: targeting the brain and lipoxygenation. Mol. Neurobiol., 42: 48-51.
- Rambjor, G.S., Walen, A.I., Windsor, S.L. and Harris, W.S. (1996): Eicosapentaenoic acid is primarily responsible for hypotriglyceridemic effect of fish oil in humans. Lipids, 31: S45?S49.
- Roth, E.M. and Harris, W.S. (2010): Fish oil for primary and secondary prevention of coronary heart disease. Curr. Atheroscler. Rep., 12: 66-72.
- Simopoulos, A.P. (2008): The importance of the omega-6/ omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp. Biol. Med., 233: 674-688.
- Sprague, M., Eldar, A., Bendiksen James, R.D, Fiona, S., Jarunan, P., Marc, H.G., Berntssen Douglas, R.T. and John, G.B. (2010): Effects of decontaminated fish oil or a fish and vegetable oil blend on persistent organic pollutant and fatty acid compositions in diet and flesh of Atlantic salmon (Salmo salar). Brit. J.Nutrit., 103: 1442-1451.
- Sprecher, H. (2000): Metabolism of highly unsaturated n-3 and n-6 fatty acids. Biochim. Biophys. Acta, 1486: 219?231.
- Young, D.S. (1990): Effects of drugs on clinical laboratory tests. 3rd AACC press; Washington DC (USA), 6 -12 p.
- Wang, Y., Botolin, D., Christian, B., Busik, J., Xu, J. and Jump, D.B. (2005): Tissue-specific, nutritional, and developmental regulation of rat fatty acid elongases. J. Lipid Res., 46: 706?715.
[Medhat Mostafa Abozid. (2018); EVALUATION OF THE EFFECT OF FEEDING ON PORTULACA OLERACEA SEED OIL ON BIOSYNTHESIS OF LONG CHAIN POLYUNSATURATED FATTY ACIDS IN RATS. Int. J. of Adv. Res. 6 (May). 306-312] (ISSN 2320-5407). www.journalijar.com
Biochemistry Department, Faculty of Agriculture, Menoufia University.