Vol. 6 (06) pp. 1183-1188 DOI: 10.21474/IJAR01/7324

MIR-378 TARGET GENE TGFB2 IN THE STAGE II COLON CANCER TISSUE.

  • Central Research Laboratory, Harran University, Sanliurfa, Turkey.
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Abstract

Colorectal cancer (CRC) is the third leading cause of cancer-related deaths worldwide. The sensitivity of markers used in the early and painless diagnosis of various cancers, including CRC, remains low. Recent studies have shown that non-protein coding small RNA molecules, called microRNAs (miRNA), play a key role in the mechanism of both the development of cancer and its treatment. In the development of certain types of cancer, including CRC, miRNAs have tumor suppressing and oncogenic effects. Therefore, the aim of the present study was to determine the oncogenic and tumor suppressing miRNAs profiles affecting cancer development and miRNA target gene in the tumor tissue as well as in normal tissues of patients with Stage II CRC. This study array analysis have demonstrated been for the first time in colorectal tumor tissues, 6 of the 8 miRNAs of the miR-378 family (hsa-miR-378i, hsa-miR-378c, hsa-miR-378d, hsa-miR-378e, hsa-miR-378f and hsa-miR-378g) showed that targets TGFB2. As a result; it is possible that early diagnosis of mir-378 colon cancer may have an important role in early diagnosis by investigating expression with TGFB2.

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References

  1. Baek, D., Villen, J., Shin, C., Camargo, F.D., Gygi, S.P., Bartel, D.P. (2008): The impact of microRNAs on protein output. Nature, 455: 7209 64-71.
  2. Corte, H., Manceau, G., Blons, H. and Laurent-Puig P., (2012). microRNA and colorectal cancer. Dig Liver Dis, 195-200.
  3. Faltejskova, P., Svoboda, M., Srutova, K., Mlcochova, J., Besse, A., Nekvindova, J., Radova, L., Fabian, P., Slaba, K., Kiss, I. (2012). Identification and functional screening of microRNAs highly deregulated in colorectal cancer. J. Cell. Mol. Med., 16:11 2655-2666.
  4. Filipowicz, W., Bhattacharyya, S.N. and Sonenberg, N. (2008). Mechanism of post-transcriptional regulation by microRNAs: are the answers in sight?. Nature Reviews Genetics, Advance online publication, 9:102-114.
  5. Gattolliat, C.H., Uguen, A., Pesson, M., Trillet, K., Brıgitte, S., Doucet, L., Robaszkiewicz, M., Corcos, L. (2015). MicroRNA and targeted mRNA expression profiling analysis in human colorectal adenomas and adenocarcinomas. European Journal of Cancer, 51: 409? 420.
  6. Grady, W.M. (2004). Genomic instability and colon cancer. Cancer Metastasis Review, 21:11-27.
  7. Gregory, R.I., Chendrimada, T.P., Cooch, N., Shiekhattar, R. (2005). Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. Cell, 123:4 631-40.
  8. Grosshansi H., and Filipowıcz, W., (2006). The expanding world of small RNAs. Nature. 451: 414-416.
  9. Hermsen, M., Postma, C., Baak, J. (2002). Colorectal adenoma to carcinoma progression follows multiple pathways of chromosomal instability. Gastroenterolgy. 123: 1109-1119.
  10. Kutter, C. and Svoboda, P. (2008). miRNA, siRNA, piRNA: Knowns of the unknown. RNA Biol., 5: 181-188.
  11. Lampropoulos, P., Zizi-Sermpetzoglou, A., Rizos, S., Kostakis, A., Nikiteas, N., Athanasios, G. and Papavassiliou, A. (2012). TGF-beta signalling in colon carcinogenesis Cancer Letters, 314: 1?7.
  12. Li, H., Dai, S., Zhen, T., Shi, H., Zhang, F., Yang, Y., Kang, L., Liang, Y., Han, A. (2014). Clinical and biological significance of miR-378a-3p and miR-378a-5p in colorectal cancer. European Journal of Cancer, 50: 1207? 1221.
  13. Lim, L.P., Lau, N.C., Garrett-Engele, P., Grimson, A., Schelter, J.M., Castle, J., Bartel, D.P., Linsley, P.S., and Johnson, J.M., (2005). Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature, 433: 769-773.
  14. Mazeh, H., Mizrahi, I., Ilyayev, N., Halle, D., Brucher, B., Bilchik, A., Proti,c M., Daumer, M., Stojadinovic, A., Avital, I. and Nissan ,A. (2013). The Diagnostic and Prognostic Role of microRNA in Colorectal Cancer-a Comprehensive review. J Cancer, 4: 281-295.
  15. Michor, F., Iwasa, Y., Lengauer, C. and Nowak, M.A. (2005). Dynamics of colorectal cancer. Semin Cancer Biol., 15(6): 484-93.
  16. Schetter, A.J., Okayama, H., Harris, C.C. (2012). The Role of MicroRNAs in Colorectal Cancer. Cancer J, 18: 244-252.
  17. Selbach, M., Schwanhausse,r B., Thierfelder, N., Fang, Z., Khanin, R. and Rajewsky, N. (2008). Widespread changes in protein synthesis induced by microRNAs.Nature. 455(7209):58-63.
  18. Shen, J., Stass, S.A. and Jiang, F. (2013). MicroRNAs as potential biomarkers in human solid tumors. Cancer Lett., 329: 125-136.
  19. Siegel, R..L, Miller, K.B., Jemal, A., (2017). Cncer Statistic, 2017. Cancer Journal for Clinicions, 67: 7-30.
  20. URL (http://kanser.gov.tr/daire-faaliyetleri/kanser-istatistikleri/2106-2014-y%C4%B1l%C4%B1-t%C3%BCrkiye-kanser-istatistikleri.html.
  21. Xu, Y. and Pasche, B. (2007). TGF-β signaling alterations and susceptibility to colorectal cancer Hum Mol Genet.,? April 15: (16) R14?R20.
  22. Wang, Y.X., Zhang, X.Y., Zhang, F.B., Yang, C,Q., Chen, X.M., and Gao, H.J., (2010). Initial study of microRNA expression profiles of colonic cancer without lymph node metastasis. Journal of Digestive Diseases, 11: 50?54.
  23. Zhang, H.Z., Zhou, H., Xiao, H.X., Li, Y. and Zhouet, T. (2014). MiR-378 is an independent prognostic factor and inhibits cell growth and invasion in colorectal cancer. Zhang et al. BMC Cancer, 14: 109.

How to Cite This Article

Cigdem gungormez. (2018); MIR-378 TARGET GENE TGFB2 IN THE STAGE II COLON CANCER TISSUE., Int. J. of Adv. Res., 6 (06), 1183-1188, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/7324

Corresponding Author

Cigdem GUNGORMEZ

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