Vol. 6 (07) pp. 1096-1102 DOI: 10.21474/IJAR01/7462

HIGH FREQUENCY SOMATIC EMBRYOGENESIS FROM COTYLEDONARY EXPLANTS OF INDIGOFERA TINCTORIA LINN.

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  • Plant Biotechnology laboratory, Department of Botany, Fatima Mata National College (Autonomous), Kollam-2, Kerala.
  • Plant Tissue culture and Molecular Biology Laboratory, Department of Botany, University of Kerala, Kariavattom- 695 581, Thiruvananthapuram, Kerala.
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Abstract

Protocol was standardized for the rapid clonal propagation through high frequency indirect somatic embryogenesis from the cotyledonary explants of a medicinally and commercially important plant, I. tinctoria Linn. Maximum embryogenic callus was induced from explants cultured on MS medium amended with 2, 4-D (0.5 mgl-1) and BA (0.5 mgl-1). Abnormal somatic embryos were developed from NAA and BA containing cultures. Among different additives or amino acids used, L-glutamine enhanced maximum high frequency embryogenic callus formation. Embryogenic calluses, when subcultured to ? MS basal medium induced maximum embryo conversion. BA or KIN along with MS or ? MS medium also enhanced the development of embryos. Maturation and rooting of somatic embryos took place in ? MS solid medium enriched with 5% sucrose. Plantlets derived from somatic embryos were hardened in sugar free liquid ? MS basal medium. Subsequent transfer of hardened plantlets to vermiculite and then to soil recorded maximum survival.

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How to Cite This Article

A. S. Rubin jose and g. M. Nair. (2018); HIGH FREQUENCY SOMATIC EMBRYOGENESIS FROM COTYLEDONARY EXPLANTS OF INDIGOFERA TINCTORIA LINN., Int. J. of Adv. Res., 6 (07), 1096-1102, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/7462

Corresponding Author

Dr. A. S. Rubin Jose
Assistant professor, Department of Botany, FMN College, Kollam, Kerala, India
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.