Vol. 6 (07) pp. 1096-1102 DOI: 10.21474/IJAR01/7462

HIGH FREQUENCY SOMATIC EMBRYOGENESIS FROM COTYLEDONARY EXPLANTS OF INDIGOFERA TINCTORIA LINN.

and
  • Plant Biotechnology laboratory, Department of Botany, Fatima Mata National College (Autonomous), Kollam-2, Kerala.
  • Plant Tissue culture and Molecular Biology Laboratory, Department of Botany, University of Kerala, Kariavattom- 695 581, Thiruvananthapuram, Kerala.
16 Downloads 88 Views
Crossref

Download Full Paper (PDF)

Abstract

Protocol was standardized for the rapid clonal propagation through high frequency indirect somatic embryogenesis from the cotyledonary explants of a medicinally and commercially important plant, I. tinctoria Linn. Maximum embryogenic callus was induced from explants cultured on MS medium amended with 2, 4-D (0.5 mgl-1) and BA (0.5 mgl-1). Abnormal somatic embryos were developed from NAA and BA containing cultures. Among different additives or amino acids used, L-glutamine enhanced maximum high frequency embryogenic callus formation. Embryogenic calluses, when subcultured to ? MS basal medium induced maximum embryo conversion. BA or KIN along with MS or ? MS medium also enhanced the development of embryos. Maturation and rooting of somatic embryos took place in ? MS solid medium enriched with 5% sucrose. Plantlets derived from somatic embryos were hardened in sugar free liquid ? MS basal medium. Subsequent transfer of hardened plantlets to vermiculite and then to soil recorded maximum survival.

Keywords

How to Cite This Article

A. S. Rubin jose and g. M. Nair. (2018); HIGH FREQUENCY SOMATIC EMBRYOGENESIS FROM COTYLEDONARY EXPLANTS OF INDIGOFERA TINCTORIA LINN., International Journal of Advanced Research (IJAR), 6 (07), 1096-1102, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/7462

Corresponding Author

Dr. A. S. Rubin Jose
Assistant professor, Department of Botany, FMN College, Kollam, Kerala, India

Article Analytics

References

  1. Arcioni A, Damiani? F, pezzotti M and Lupotto E (1990) Alfa alfa, Luerene (Medicago) In: Bajaj Y P S (eds) Biotechnology in Agriculture and Forestry 10- Legumes and Oil seed Crops I (pp. 197-241). Springer-Verlag, Berlin.
  2. Epstein E (1972) Mineral nutrition of plants ? Principles and perspectives. John Wiley, New York.
  3. Berthouly M & Michaux-Ferriere N M (1996) High frequency Somatic embryo genesis in Coffea canephora- induction conditions and histological evolution in: A Fujiwara (ed.) Plant Tissue culture 1982 (pp 101-103). Maruzen, Tokyo
  4. Etienne H, Sotta B, Montoro P, Miginiac E & Carron M-P (1993) Relations between exogenous growth regulators and endogenous indole-3-aceticacid and abscic acid in the expression of somatic embryogenesis in Hevea brasiliensis (Mull.Arg.). Plant Sci. 88:91-96
  5. Gomes da Cunha A C & Ferreira M F (1996) Somatic embryogenesis, organogenesis and callus growth kinetics of flax. Plant Cell Tiss. Org. Cult. 47: 1-8
  6. Guerra M P & Handro W (1998) Somatic embryogenesis and plant regeneration in different organs of Euterpe edulis (Palmae): Control and structural features. J. Plant Res. 111: 65-71
  7. Komamine A, Kawahara R, Matsumoto M, Sunabori S, Toya T, Fujiwara A, Tsukahara M, Smith J, Ito M, Fukuda H, Nomura K, & Fujimura T (1992). Mechanisms of Somatic embryogenesis in cell cultures: physiology, biochemistry and molecular biology. In Vitro Cell Dev. Biol. Plant 28P: 11-14
  8. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays
  9. organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng. Plant Cell Rep. 19: 727-732
  10. Parrot W A, Bailey M A, Durham R E & Mathews H V (1992) Tissue culture and regeneration of legumes. In: Moss J P (ed) Proc Int Workshop Biotechnol Crop Improv Asia- ICRISAT. Hyderabad, India pp 115-148
  11. Plant Cell Tiss. Org. Cult. 44: 169-176
  12. Sofiari E, Raemakers C J J M, Kunju E, Danso K & Van Lammeren A M (1997) Comparison of NAA and 2, 4- D induced somatic embryogenesis in Cassava. Plant Cell Tiss. Org. Cult. 50: 45-56
  13. Strandberg J O (1993) Meristem culture of Ophiopogon japonicus and production of embryo-like structures. Plant Cell Tiss. Org. Cult. 32: 277-282
  14. Stuart D A & Strickland S G ( 1984) Somatic embryogenesis from cell cultures of Medicago sativa I. The role of the amino acid addition to the regeneration medium. Plant Sci. Lett. 34: 165-174
  15. Tang W (2000) High frequency plant regeneration via somatic embryogenesis and
  16. organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng. Plant Cell Rep. 19: 727-732
  17. Vasil I K (1982) Somatic embryogenesis and plant regeneration in cereals and grasses
  18. with tobacco tissue cultures. Plant. 15: 473-497.
  19. Venkataiah P, Bhanuprakash P, Kalyan S S, Subhash? , (2016). Somatic? embryogenesis and plant regeneration of Capsicum baccatum L.,J.Genetic Eng.? and Biotech.14: 1 Pp. 55-60.
  20. Xie D Y and Hong Y (2000) Regeneration of Acacia mangium through somatic embryogenesis. Plant Cell Rep. 20: 34-40.
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.