Vol. 6 (07) pp. 1114-1122 DOI: 10.21474/IJAR01/7465

STUDYING THE SOCIO-DEMOGRAPHIC PROFILE AND THE MOLECULAR BASES OF THALASSEMIA IN BASRAH/ IRAQ.

, , , and
  • Department of Medical Laboratory Technology, College of Health and Medical Technology, Southern Technical University, Basrah / Iraq.
10 Downloads 74 Views
Crossref

Download Full Paper (PDF)

Abstract

The thalassemia is one of the most common inherited diseases worldwide with considerable frequencies in the Middle East region, including Iraq. As the disease requires long-term care, an establishment of an effective preventative program creates a major armament in management. As part of this effort, we established a primary study to determine the types of thalassemic patients based on family medical history, age, region, blood groups and consanguineous marriages. The results showed that the recruited patients (120) were distributed in nine different regions of Basrah province with high percentage (43.33%) in central city with age 8-12 years (30.7%), O+ blood group (40%) and 80.83 % of the investigated patients had consanguineous marriages. Based on the present findings, the molecular basis of fifty four thalassemic patients were investigated, using ARMS-PCR (Amplification Refractory Mutation System), to determine the most common types of thalassemia mutations in the region. Seven mutation types were detected (IVS1nt.5, Codon 8/9, Codon -88, Codon 8, Codon 15 Asia Indian, Codon 41/42, Codon 30). The results showed that codons 30, -88 and IVS Int 5.mutations were the highest frequent compared with other mutations studied in the thalassemic samples.

Keywords

Article Analytics

References

  1. Modell B &Darlison M (2008). Global epidemiology of hemoglobin Disorders and derived service indicators. Bull World Hlth Org 86; 480-487.
  2. Weatherall, D.J. (2006). The Thalassemias: Disorders of Globine Synthesis. 7th ed. New York: McGrow Hill. 633-66.
  3. Mok,S.; Imwong, M.and Mackinnon, M. J. (2011). Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription. BMC Genomics.12; 391
  4. ChristiansonA, HowsonCP, ModellB (2006) .March of Dimes Global Report on Birth Defects. New York, NY: March of Dimes Birth Defects Foundation.
  5. Chan, V.; Chan, V.W., Tang, M., Lau, K., Todd, D.,et al. (1997). Molecular defects in Hb H hydropsfetalis. Br J Haematol, 96 (2); 224-228.
  6. Chui, D.H.K. & Waye, J.S. (1998). HydropsFetalis Caused by -Thalassemia: An Emerging Health Care Problem. Blood, 91; 2213 - 2222.
  7. Weatherall DJ and Clegg JB. 2001. The thalassaemia syndromes. 4th Blackwell Science, Oxford
  8. Thein, SL. (2004). Genetic insights into the clinical diversity of betathalassaemia. Br J Haematol. 124 (3); 264-74.
  9. Li, Q., Li, LY. and Huang, SW. (2008). Rapid genotyping of knownmutations and polymorphisms in beta-globin gene based on theDHPLC profile patterns of homoduplexes and heteroduplexes. Clin Biochem.41; 681-687.
  10. Al-Abboodi, M.J. (2011). Molecular Study for Some Beta-globinMutations in Sample of Iraqi Thalassemic Patients. MSc thesis,Institute of Genetic Engineering and Biotechnology for Post GraduateStudies, University of Baghdad, Iraq.
  11. Al-Allawi,NA; Jubrael, JM. and Hughson, M.(2006). Molecularcharacterization of beta-thalassemia in the Dohuk region of Iraq. Hemoglobin. 30 (4); 479-486.
  12. Al-Assadi, Z.M. (2007). Molecular detection of some mutationsassociated with beta-thalassemia in Iraq.Ph.D. thesis, Institute ofGenetic Engineering and Biotechnology for Post Graduate Studies,University of Baghdad, Iraq.
  13. Al-Hadad, R.H. (2007). Molecular Genetic Study of β-thalassemiamajor syndrome in Baghdad. MS.c thesis, Al-Nahrain University,Iraq.
  14. Al-Jasha'mi, J.N. (2008). Identification of Some Beta-Thalassemia Mutations in Iraqi Population MSc thesis, Institute of Genetic.
  15. Khalid G .Al-Fartosi1 and Hanaa Ali Azez (2014). Mutation Which Causes β- thalassemia in AL-Muthanna Province. International Journal of Advanced Research,? 2 (10); 321-329
  16. Stephen, A. (2002). Mutation detection by ARMS/RFLP,pyrosequencing, oligohybridization, oligo ligation assay.MRC pathself-help course (London).
  17. Talmaci, R.; Traeger, J.; Kanavakis, E.; Coriu, D., Colita D. andGavrila L. (2004). Scanning of β -globin gene for identification of β-thalassemia mutation in Romanian population. Cell. Mol.Med., 2; 232-240.
  18. Baig, S.M.; Rabbi, F.; Hameed, U.;Qureshi, J.A.; Mahmood, Z.;Bokhari, S.H.; Kiani, A., Hassan, H.; Baig, J.M.;Azhar, A.andZamanT.( 2005).
  19. Molecular characterization of mutations causing β -thalassemia in Faisalabad Pakistan using the amplification refractorymutation system (ARMS-PCR). National Institute for Biotechnology and Genetic Engineering (NIBGE). 11 (2); 80-83.
  20. Basak, A. N. (2007). The molecular pathology of β-thalassemia in Turkey. Hemoglobin, 31 (2); 233?241.
  21. Mirasena, S.; Shimbhu, D.; Sanguansermsri, M. and Sanguansermsri,T. (2008). Detection of β-thalassemia mutations using a multiplexamplification refractory mutation system assay. Hemoglobin. 32 (4); 403-409.
  22. Sarookhani,M. R.; Ahmadi, M.H.andAmirizade, N. (2009).Molecular Spectrum of Beta-Globin Mutations in TransfusionDependent Patients with Thalassemia in Qazvin Province, I. J.Med. Sci .34 (1); 17-22.
  23. Saleh-Gohari,N.and Bazrafshani MR. (2010). Distribution of β-Globin Gene Mutations in Thalassemia Minor Population of KermanProvince, Iran.Iranian J Publ Health. 39 (2); 69-76.
  24. Sambrook,J.; Fritsch, E.F. and Maniatis,T. (1989). Molecularcloning: a laboratory manual. 2nd Ed., Cold spring harbor laboratorypress, cold spring harbor, New York.
  25. Chan, O.T.; Westover, K.D.; Dietz, L.; Zehnder, J.L. and Schrijver, I. (2010). Comprehensive and efficient HBB mutation analysis fordetection of beta-hemoglobinopathies in a pan-ethnic population. J. Clin. Pathol. 133 (5); 700-707.
  26. Saud, A .M.; Al-Azzaiwe, F .H. and Al-Kazaz, A.A.(2013). Molecular and Biochemical study on β-Thalassemia Patients In Iraq. PHD thesis. College of science .Baghdad University.

How to Cite This Article

Khairallah A S Mohammed, Majid A. Maatook, Hussam S. Aziz, Ali M. Afat and Sarah Hani. (2018); STUDYING THE SOCIO-DEMOGRAPHIC PROFILE AND THE MOLECULAR BASES OF THALASSEMIA IN BASRAH/ IRAQ., Int. J. of Adv. Res., 6 (07), 1114-1122, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/7465

Corresponding Author

Dr. Khairallah A S Mohammed
Department of Medical Laboratory Technology, College of Health and Medical Technology, Southern Technical University, Basrah / Iraq
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.