17Sep 2018

16S RRNA CHARACTERIZATION OF CELLULASE PRODUCING BACILLUS CEREUS PREVALENT IN DECAYING AGRO WASTE.

  • Department of Botany, BRABU, Muzaffarpur, Bihar, India.
  • Department of Botany, T.P.S College, Magadh University, Bihar, India.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

The present study was undertaken to isolate and screen an efficient cellulase producing bacteria from decaying agricultural waste. Bacterial cultures were isolated from agricultural wastes produced in the Bihar region. The preliminary isolation was done on nutrient agar media. The isolates obtained were screened for cellulase production by plating on CMC and Congo red agar media to observe the zone of cellulose hydrolysis. Out of 25 isolates 14 strains showed cellulase production on CMC and congo red agar. Further these isolates were used for crude enzyme production. The carboxy methyl cellulase (CMCase) activity was assayed using a method described by Miller, with some modifications. S18 bacterial isolate which showed maximum enzyme activity (0.420 IU/ml/min) was further subjected to biochemical and molecular characterization. On the basis of molecular characterization, the isolates S18 was identified as Bacillus cereus. Therefore, the cellulase producing Bacillus cereus isolated and identified in this study from Bihar region have potential for further use and study, such as to isolate novel cellulases with unique characteristics. Also the enzymatic degradation of cellulose agrowaste produced in this region has been suggested as a possible alternative for the conversion of lignocelluloses substrate into fermentable sugars.

  1. Afzal I., Shah A. A., Makhdum Z., Hameed A., Hasan F, 2012. Isolation and characterization of cellulase producing Bacillus cereus MRLB1 from soil. Minerva Biotecnologica September, 24(3):101-9
  2. Aristidou A. and M. Penttila, 2000. Metabolic engineering applications to renewable resource utilization, Current Opinion in Biotechnology,11(2):87-98
  3. Celenk Molva, Mert Sudagidan, Burcu Okuklu, 2009. Extracellular enzyme production and enterotoxigenic gene profiles of Bacillus cereus and Bacillus thuringiensis strains isolated from cheese in Turkey, Food Control 20 :829- 834
  4. Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O., 2008. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res.1:3-6.
  5. Edgar RC, 2004: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res, 32(5):1792-1797.
  6. Gurtler, V. and Stanisich, V. A, 1996. New approaches to typing and identification of bacteria using the 16s-23s rDNA spacer region. Microbiology, 142, 3-16.
  7. Lynd L. R., Weimer, P. J., van Zyl, W. H. and Pretorius, I. S., 2002. Microbial cellulose utilization: Fundamentals and biotechnology, Microbiology and Molecular Biology Reviews, 66(3), 506- 577
  8. Maki M, Leung KT, Qin W., 2009. The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass. Int J Biol Sci.; 5:500-516.
  9. Maier RM, Peper IL, Gerba CP, 2000. Environment Microbiology, Academic Press.
  10. Maki ML, Broere M ,Leung KT and Qin W., 2011 Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers. Int J Biochem Mol Biol. 2(2): 146?154
  11. Mawadza, C., Hatti-Kaul, R., Zvauya, R. and Mattiasson, B.,2000. Purification and characterization of cellulases produced by two Bacillus strains. J Biotechnol 83:177 187.
  12. Miller G. L, 1959.Use of dinitrosalicylic acid reagent for determination of reducing sugar, Analytical Chemistry, vol. 31, no. 3, pp. 426 -428.
  13. Muhammad Irfan, AsmaSafdar, Quratulain Syed, Muhammad Nadeem, 2012. Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity. Turk J Biochem, 37 (3): 287- 293
  14. Mukesh Kumar D. J, Poovai C. L, Puneeth Kumar, Sushma Saroja Y, Manimaran A. and Kalaichelvan P. T, 2012. Optimization of Bacillus cereus MRK1 cellulase production and its Biostoning activity, Der Pharmacia Lettre, 4 (3):881-888
  15. Nakamura K, Kappamura K., 1982. Isolation and identification of crystalline cellulose hydrolyzing bacterium and its enzymatic properties. J Ferment Technol, 60 (4): 343-8.
  16. Oberoi, H.S.; Yogita, C.; Sunil B.; Gurpreet, S. D., (2008). Production of cellulose through solid state fermentation using kinnow pulp as a major substrate. Food Bioprocess. Technol., 3(4),528-536.
  17. Patagundi BI, Shivasharan CT, Kaliwal BB., 2014.Isolation and characterization of cellulase producing bacteria from soil, International Journal of Current Microbiology and Applied Sciences; 3(5):59-69.
  18. Perez J, Munoz-Dorado J, de la Rubia T, Martinez J, 2002. Biodegradation and biological treatments of cellulose, hemicelluloses and lignin: an overview. Int Microbiol; 5 (2): 53 - 63.
  19. Philip J, Tanuja T, Bedi S., 2016. Occurrence of Cellulose Degraders in Fruit and Vegetable Decaying Wastes. J Bioremediat Biodegrad 7: 373. doi: 10.4172/2155-6199.1000373
  20. ShohamY, Lamed R, Bayer EA., 1999. The cellulosome concept as an efficient microbial strategy for the degradation of insoluble polysaccharides. Trends Microbiol 7(7):275 -281.
  21. Schulein M,1998. Cellulases of Trichoderma reesei. In: Wodd WA, Abelson JN (eds) Methods in enzymology, vol 160. Academic, New York, pp 234?242
  22. Sonia Sethi, Aparna Datta, B. Lal Gupta, and Saksham Gupta, 2013. Optimization of Cellulase Production from Bacteria Isolated from Soil. Hindawi Publishing Corporation, ISRN Biotechnology, ArticleID985685)
  23. Tanuja and Philip J, 2017. Molecular Characterization of Cellulase Producing Microbacterium Barkeri Isolated from Agricultural Waste. Int. J. Adv. Res. 5(8), 1344-1349
  24. Venkata Naga Raju E, Goli Divakar, T. Rajesh, Akram Ghazi, Asra Pourgharashi., 2013. Screening and isolation of cellulase producing Bacteria from dump yards of vegetable wastes. World journal of pharmacy and pharmaceutical res. 3(1): 428-435.

[Jaya Philip and Tanuja. (2018); 16S RRNA CHARACTERIZATION OF CELLULASE PRODUCING BACILLUS CEREUS PREVALENT IN DECAYING AGRO WASTE. Int. J. of Adv. Res. 6 (Sep). 473-478] (ISSN 2320-5407). www.journalijar.com

Jaya philip
Department of Botany, BRABU, Muzaffarpur, Bihar, India

DOI:

Article DOI: 10.21474/IJAR01/7699      
DOI URL: https://dx.doi.org/10.21474/IJAR01/7699

Download Full Paper

Download PDF No. of Downloads: 21 | No. of Views: 64