Detection and Purification of Panton – Valentine Leukocidin toxin Produced by Methicillin resistant Staphylococcus aureus

Panton - Valentine leukocidin (PVL) produced by community acquired methicillin Staphyloccous aureus involved in skin and soft tissue infection comprised of two fraction namely PVLS and PVLF . In the present study , one of aims was to identify resistance profile for MRSA, and other aims are detection and purification of PVL toxin. A total of (100) MRSA isolates were recovered from hospitalized patients in Baghdad in 2013 . The percentage of PVL – positive was represented by 27% of isolates and 55.6% of them isolated from wound and 40.7% of them from abscess . All isolates were resistant to cloxacillin , followed by cefoxitin and cephalexin and lincomycin (86, 51 and 23)% respectively. Result of PVL toxin purified by sequatinal ammonium sulphate precipitation ion exchange and gel filtration with sepharos 6B then hydroxyl aptite chromatography revealed the protein concentration was 22.4 µg/ml with molecular weight 35.4kDa.