Vol. 6 (10) pp. 615-620 DOI: 10.21474/IJAR01/7847

COMPARISON BETWEEN ESTRADIOL AND GNRH-CIDR BASED PROGRAMS ON SUPER OVULATION IN HOLSTEIN COWS/HEIFERS.

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  • Diagnostic Imaging and Endoscopy Unite, Animal Reproduction Research Institute, Al Haram.
  • Department of Theriogenology, Faculty of Veterinary Medicine, Alexandria University.
  • Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, P.O: 13736, Tokh, Kaliobia, Egypt.
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Abstract

Objectives: The aim of the current study is to compare the response of GnRH-CIDR and estradiol-CIDR based programs on super ovulation outcomes in Holstein cows. Methods: 12 Holstein cows/heifers were divided into two groups. Groups (A/N=7) synchronized with GnRH and group (B/N=5) synchronized with Estradiol. Both treated groups received progesterone releasing intravaginal device (CIDR) at day (0) and removed at day (7). Group (A) received GnRH (Receptal? /10 ?g) at day (2 and 8) and Group (B) received estradiol benzoate at day (0). Both groups were super stimulated with Follicle-stimulating hormone (Follitropin-V) twice daily from day (4) to day (7). Cloprostenol (Estrumate?/500?g) was administrated at a day (7PM& 8 PM) in group (A) and at day (6AM&PM) for group(B). Cows/heifers were subjected to artificial insemination at day (9AM&PMand 8PM&9AM) for group (A) and (B) respectively. The embryo flushing was done at day (16) for group (A) and at day (15) for group (B). Results: The total number of recovered embryos was better in group (A/55) than group (B/45); although the recovered embryos per flush was 7.86 for group (A) which was lower than that of group (B/9). Even though, the embryo quality was better in group (A) as follow: 47.2% embryo grade I , 21.8% embryo grade II, 12.7% embryo grade III, 18.1% embryo grade IV, while they were 33.3%, 28.8%,11.1% and 26.6% respectively in group (B). The pregnancy rate in the recipient heifers were 30.90% and 24.44% for group (A) and (B) treated donors respectively. Conclusion: GnRH-CIDR based program was better than estradiol-CIDRbased programon super ovulation in Holstein cows especially in terms of embryo quality, and pregnancy rate.

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References

  1. Adams, G.P. (1994): Control of ovarian follicular wave dynamics in cattle: implications for synchronization &superstimulation. Theriogenology, 41(1): 19-24.
  2. Andrade, J.C.I., Oliveira, M.A., Lima, P.F., Guido, S.I., Bartolomeu, C.C., Ten?rioFilho, F., Pina, V.M., Iunes-Souza, T.C., Paula, N.R., Freitas, J.C. (2003): The use of steroid hormones in superovulation of Nelore donors at different stages of estrous cycle. Anim. Reprod. Sci., 77(1-2): 117-125.
  3. Andrade, J.C.I., Oliveira, M.A., Lima, P.F., Santos Filho, A.S. and Pina, V.M. (2002): Use of steroid hormone treatments prior to superovulation in Nelore donors. Anim. Reprod. Sci., 69(1-2): 9-14.
  4. Baracaldo, M.I., Martinez, M.F., Adams, G.P. and Mapletoft, R.J. (2000): Superovulatory response following transvaginal follicle ablation in cattle. Theriogenology, 53(6): 1239-1250.
  5. Bo, G.A., Adams, G.P., Pierson, R.A. and Mapletoft, R.J. (1996): Effect of progestogen plus estradiol-17β treatment on superovulatory response in beef cattle. Theriogenology, 45(5): 897-910.
  6. Bo, G.A., Adams, G.P., Pierson, R.A. and Mapletoft, R.J. (1995): Exogenous control of follicular wave emergence in cattle. Theriogenology, 43(1): 31-40.
  7. B?, G.A., Guerrero, D.C., Tr?bulo, A., Tr?bulo, H., Tr?bulo, R., Rogan, D., Mapletoft, R.J. (2010): New approaches to superovulation in the cow. Reprod. Fertil. Dev., 22(1):106-112.
  8. B?, G.A. and Mapletoft, R.J. (2014): Historical perspectives and recent research on superovulation in cattle. Theriogenology, 81(1): 38?48.
  9. Bo, G.A.,?Adams, G.P., Nasser, L.F., Pierson, R.A. and Mapletoft, R.J. (1993): Effect of estradiol valerate on ovarian follicles, emergence of follicular waves and circulating gonadotropins in heifers. Theriogenology, 40(2): 225-239.
  10. Bungartz, L. and Niemann, H. (1994): Assessment of the presence of a dominant follicle and selection of dairy cows suitable for superovulation by a single ultrasound examination. J. Reprod. Fertil.,101(3): 583-591.
  11. Caccia, M. and B?, G.A. (1998): Follicle wave emergence following treatment of CIDR-B implanted beef cows with estradiol benzoate and progesterone. Theriogenology, 1(49): 341.
  12. Colazo, M.G., Mart?nez, M.F., Small, J.A., Kastelic, J.P. and Mapletoft, R.J. (2005): Effect of estradiol valerate on ovarian follicle dynamics and superovulatory response in progestin-treated cattle. Theriogenology, 63(5): 1454-1468.
  13. Cushman, R.A., DeSouza, J.C., Hedgpeth, V.S. and Britt, J.H. (1999): Superovulatory response of one ovary is related to the micro-and macroscopic population of follicles in the contralateral ovary of the cow. Biol. Reprod., 60(2): 349-354.
  14. Edmonson, A.J., Lean, I.J., Weaver, L.D., Farver, T. and Webster, G. (1989): A body condition scoring chart for Holstein dairy cows. J. dairy sci., 72(1): 68-78.
  15. Ginther, O.J., Knopf, L. and Kastelic, J.P. (1989): Temporal associations among ovarian events in cattle during oestrous cycles with two and three follicular waves. J. Reprod. Fertil., 87(1): 223-230.
  16. Hahn, J. (1992): Attempts to explain and reduce variability of superovulation. Theriogenology, 38(2): 269-275.
  17. Kim, I.H., Son, D.S., Yeon, S.H., Choi, S.H. and Yoon, J.T. (2001): Effect of dominant follicle removal before superstimulation of follicular growth, ovulation and embryo production in Holstein cows. Theriogenology, 55(4): 937-945.
  18. Kim, U.H., Suh, G.H., Nam, H.W., Kang, H.G. and Kim, I.H. (2005): Follicular wave emergence, luteal function and synchrony of ovulation following GnRH or estradiol benzoate in a CIDR-treated, lactating Holstein cows. Theriogenology, 63(1): 260-268.
  19. Mapletoft, R.J., Steward, K.B. and Adams, G.P. (2002): Recent advances in the superovulation in cattle. Reprod. Nutr. Deve.,42(6): 601-611.
  20. Mitchell, B.R., Martinez, M., Bentley, D.M., Mapletoft, R.J. (1998): A comparison of estradiol 17β and GnRH in synchronizing follicle wave emergence on superovulatory response in Holstein cows. Theriogenology, 49 (1): 380.
  21. Monniaux, D., Chupin, D. and Saumande, J. (1983): Superovulatory responses of cattle. Theriogenology, 19(1): 55-81.
  22. Nasser, L.F., Adams, G.P., Bo, G.A. and Mapletoft, R.J. (1993): Ovarian superstimulatory response relative to follicular wave emergence in heifers. Theriogenology, 40(4): 713-724.
  23. Nogueira, M.F.G., Barros, B.J.P., Teixeira, A.B., Trinca, L.A., and Barros, C.M. (2002): Embryo recovery and pregnancy rates after the delay of ovulation and fixed time insemination in superstimulated beef cows. Theriogenology, 57(6): 1625-1634.
  24. Pursley, J.R., Mee, M.O. and Wiltbank, M.C. (1995): Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology, 44(7): 915-923.
  25. Wright, J.M. (1998): Photographic illustrations of embryo developmental stage and quality codes. In: Stringfellow DA, Siedel SM (eds) Manual of the International Embryo Transfer Society (3rded). IETS, Savoy, Illinois, pp. 167-170
  26. Yaakub, H., Duffy, P., O'Callaghan, D. and Boland, M.P. (1998): Effect of timing of oestradiol benzoate injection relative to gonadotropin treatment on superovulatory response, and on embryo yield and quality in beef heifers. Anim. Reprod. Sci., 52(3): 191-204.

How to Cite This Article

Oshba MR, Sosa GAM, Nossier MB, Fadel MS and El-Raey M. (2018); COMPARISON BETWEEN ESTRADIOL AND GNRH-CIDR BASED PROGRAMS ON SUPER OVULATION IN HOLSTEIN COWS/HEIFERS., Int. J. of Adv. Res., 6 (10), 615-620, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/7847

Corresponding Author

Mohamed El-Raey Mohamed
Ass.Prof. of Theriogenology, Faculty of Veterinary Medicine, Benha University
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.