Vol. 6 (10) pp. 621-629 DOI: 10.21474/IJAR01/7848

Effect of Oxygen Concentration on Development and Quality of Embryos during Intracytoplasmic Sperm Injection (ICSI).

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  • Department of obstetrics and gynecology, Ain Shams University,Cairo,Egypt.
  • Department of Animal bioteсһnologү, Genetic Engineering and Bioteсһnologү ReѕearсһInѕtitute (GEBRI), Ѕadat Сitү Univerѕitү, Egypt.
  • Department of Community, environmental & industrial health, Ain Shams University, Cairo,Egypt.
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Abstract

Background: Elevated and non-physiological O2 concentrations could create unfavorable conditions in the substrate to produce free oxygen radicals that cause oxidative stress, which may be involved in the etiology of defective embryo development with higher rates of fragmentation. Aim of the Work: to evaluate the effects of oxygen tension on the early development and quality of embryos during Intracytoplasmic sperm injection (ICSI) treatment cycles. Patients and Methods: This prospective cohort observational study was carried out in the Embryology Laboratory of Assisted Reproduction Unit at Ain Shams University Maternity Hospital in the period from Octoberof2014 to October of 2017.This study comprised 1143 female gametes (oocytes) from 100 ICSI cycles patients. All the oocytes were injected by the same embryologist and were divided after injection equally into two groups according to system of incubation. Results: Fertilization and division Rates were significantly lower among CO2 incubator group than among Triplet incubator group, No significant difference between Implantation Rate of cases underwent incubation at atmospheric versus low oxygen tension and versus mix of both, No significant difference between outcomes in cases underwent incubation at atmospheric versus low oxygen tension and versus mix of both. Conclusion: Our findings support the idea that human embryo culture in low oxygen tension will improve the fertilization and embryo development, and ART outcomes.

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References

  1. Sobrinho DB, Oliveira JB, Petersen CG, Mauri AL, Silva LF, Massaro FC, Baruffi RL, Cavagna M, Franco JG. IVF/ICSI outcomes after culture of human embryos at low oxygen tension: a meta-analysis. Reproductive Biology and Endocrinology. 2011;9(1):143.
  2. Wale PL, Gardner DK.The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction.Human reproduction update. 2015;22(1):2-2.
  3. Rinaudo PF, Giritharan G, Talbi S, Dobson AT, Schultz RM. Effects of oxygen tension on gene expression in preimplantation mouse embryos.FertilSteril 2006, 86:1252-1265, 1265 e1251-1236.
  4. Yuan YQ, Van Soom A, Coopman FO, Mintiens K, Boerjan ML, Van Zeveren A, de Kruif A, Peelman LJ: Influence of oxygen tension on apoptosis and hatching in bovine embryos cultured in vitro. Theriogenology 2003, 59:1585-1596.
  5. Guerin P, El Mouatassim S, Menezo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod update 2001, 7:175-189.
  6. Kang JT, Atikuzzaman M, Kwon DK, Park SJ, Kim SJ, Moon JH, Koo OJ, Jang G, Lee BC.Developmental competence of porcine oocytes after in vitro maturation and in vitro culture under different oxygen concentrations.Zygote. 2012;20(1):1-8.
  7. Kovacic B, Vlaisavljevic V: Influence of atmospheric versus reduced oxygen concentration on development of human blastocysts in vitro: a prospective study on sibling oocytes. Reprod Biomed Online 2008, 17:229-236.
  8. Edwards NA, Watson AJ, Betts DH. P66Shc, a key regulator of metabolism and mitochondrial ROS production, is dysregulated by mouse embryo culture. MHR: Basic science of reproductive medicine. 2016;22(9):634-47.
  9. Kovačič B, Sajko MČ, Vlaisavljević V.A prospective, randomized trial on the effect of atmospheric vers?us reduced oxygen concentration on the outcome of intracytoplasmic sperm injection cycles.Fertility and sterility. 2010;94(2):511-9.
  10. Yang CS, Zhang J, Zhang L, Huang J, Wang Y. Mechanisms of body weight reduction and metabolic syndrome alleviation by tea. Molecular nutrition & food research. 2016;60(1):160-74.?
  11. Waldenstrom U, Engstrom AB, Hellberg D, Nilsson S. Low-oxygen compared with high-oxygen atmosphere in blastocyst culture, a prospective randomized study. FertilSteril 2009, 91:2461-2465.
  12. Kovacic B, Sajko MC, Vlaisavljevic V. A prospective, randomized trial on the effect of atmospheric versus reduced oxygen concentration on the outcome of intracytoplasmic sperm injection cycles.FertilSteril 2010, 94:511-519.

13.??? 13.Calzi F1,?Papaleo E,?Rabellotti E,?Ottolina J,?Vailati S,?Vigan? P,?Candiani M.Exposure of embryos to oxygen at low concentration in a cleavage stage transfer program: reproductive outcomes in a time-series analysis;2012

  1. Chambers GM, Chughtai AA, Farquhar CM, Wang YA, Risk of preterm birth after blastocyst embryo transfer: a large population study using contemporary registry data from Australia and New Zealand. Fertil.Steril.104, 997?1003.
  2. Christianson, M.S., Zhao, Y., Shoham, G., Granot, I., Safran, A., Khafagy, A., Leong, M., Shoham, Z., Embryo catheter loading and embryo culture techniques: results of a worldwide Webbased survey. J. Assist. Reprod. Genet. 31, 1029?1036.
  3. Catt J and Henman M. Human Reproduction, 2000; Vol. 15, (Suppl. 2), pp. 199-206.
  4. Cohen J, Gilligan A, Esposito W, Schimmel T, Dale B. Ambient air and its potential effects on conception in vitro. Hum Reprod 1997;12:1742?9.
  5. Peng ZF, Shi SL, Jin HX, Yao GD, Wang EY, Yang HY, Song WY, Sun YP. Impact of oxygen concentrations on fertilization, cleavage, implantation, and pregnancy rates of in vitro generated human embryos. International journal of clinical and experimental medicine. 2015;8(4):6179.
  6. Guo, N., Li, Yu., Ai, J., Gu, L., Chen, W., and Liu, Q., J. Clin. Exp. Pathol., 2014, vol. 7, no. 9, pp. 6191?6198.
  7. Wale PL and Gardner DK. Time-lapse analysis of mouse embryo development in oxygen gra?dients.Reprod Biomed Online 2010; 21: 402-410.
  8. Bedaiwy MA, Falcone T, Mohamed MS, Aleem AA, Sharma RK, Worley SE, Thornton J and Agarwal A. Differential growth of human embryos in vitro: role of reactive oxygen species. FertilSteril2004;82, 593-600.
  9. Fischer B, Bavister BD 1993 Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. Journal of Reproductionand Fertility 99, 673?679.
  10. Trokoudes KM, Minbattiwalla MB, Kalogirou L. Controlled natural cycle IVF with antagonist use and blastocyst transfer. Reprod Biomed Online 2005; 11: 685-689.
  11. Bavister B. Oxygen concentration and preim?plantation development. Reprod Biomed Online 2004; 9: 484-486.

How to Cite This Article

Azza A. Abdel Razek, Hayam S. Foad, Hazem M. EL-Hariri, Gamal F. Mustafa and Omaima A. Khamiss. (2018); Effect of Oxygen Concentration on Development and Quality of Embryos during Intracytoplasmic Sperm Injection (ICSI)., Int. J. of Adv. Res., 6 (10), 621-629, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/7848

Corresponding Author

Azza A. Abdel Razek
Department of obstetrics and gynecology, Ain Shams University,Cairo,Egypt
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.