EFFECT OF SPERMIDINE (SPD) ON PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES IN ZEA MAYS L. UNDER UV-B STRESS.
- Centre for Research and Postgraduate Studies in Botany, AyyaNadarJanakiAmmal College (Autonomous ), Sivakasi, Tamil Nadu, India.
- Department of Botany with specialization in Plant Biotechnology, The Standard Fireworks Rajaratnam College for Women, Sivakasi, Tamil Nadu, India.
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Ultraviolet-B (UV-B) radiation is one of the most important abiotic stress factors that could influence plant growth, development and productivity. Spermidine (Spd) is an important plant growth regulator involved in a wide variety of physiological processes. The study was carried out to investigate the protective role of exogenous on alleviation of UV-B stress in Zea mays L. Our result showed that UV-B stress significantly decreased the growth parameters, total soluble sugars, aminoacid, proline content, superoxide dismutase (SOD), catalase (CAT), peroxidase activity (PD) and Malondialdehyde content (MDA) in both the seedlings, but the combination of Spd pretreatment and UV-B enhanced the vegetative growth characters such as shoot and root length, shoot and root fresh weights and dry weights, photosynthetic pigment, non photosynthetic pigment composition and total soluble protein, total soluble glucose, free amino acid, SOD, CAT, PD, MDA and NR activities. However, UV-B stress induced positive effects on growth characters and biochemical constituents. Therefore appropriate concentration of Spd could be used as a simple, practical and inexpensive method for modulating the effects of UV-B stress in plants.
- Aworski, E.G. (1971). Nitrate reductase activity assay in intact plant tissue. Biochem. Biophys. Res. Commun. 43: 1274-1279 Plantation and Natural forest in Amarkantak. Indian Forester.? 2: 240 ? 250.
- Baron, K., Stasolla, C. (2008). The role of polyamines during in vivo and in vitro development. In vitro cellular & Developmental. Biology- Plant.? 44: 384-395.
- Bates, L.S., Waldren, R.P., and Teare, I.T. (1973). Rapid determination of proline for water stress studies. Plant Sci. 2: 205-207.
- Bowler, C.,Van Montagu, M. and Inze, D. (1992). Superoxide dismutase and stress tolerance. Plant Physiol. Plant Mol. Biol. 43: 83?116.
- Burritt, D.J. (2008). The polycylic aromatic hydrocarbon phenanthrene causes oxidative stress and alters polyamine metabolism in the aquatic liverwort Ricciafluitans L. Plant Cell & Environment. 31: 1416-1431.
- Chattopadhayay, K., Tiwari, B.S., Chattopadhayay, G.,? Bose, A., Sengupta, D.N., Ghosh, B. (2002).? Protective? role of exogenous polyamines on salinity stressed rice (Oryza sativa)? plants. Physiol. Plant. 116: 192-199.
- Duran, H.G., Shu, Y., Liu, D.H., Qin, D.H., Liang, H.G., Lin, H.H. (2006). Effects of exogenous spermidine on photosystemII of wheat seedlings under water stress. J. Integr. Plant Biol. 48: 920- 927.
- Heath, R.L. and Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and bio 125: 189-198.
- Hunter, K., Burritt, D.J. (2005). Light quality influences the polyamine? content of lettuce (Lactuca sativa L.) cotyledons explants during? shoot production in vitro. Plant Growth Regulation.? 45:53-61.
- Igarashi, K., Kashiwagi, K. (2000). Mysterious modulators of cellular function. Boichemical&Biophysical? Research Communications. 271: 559-564.
- Jayaraman, J. (1981). Laboratory Manual in biochemistry, Wiley Eastern Limited, Madras. 180.
- Kusano, T., Berberich, T., Tateda, C.,Takahashi, Y. (2008). Polyamines essential factors for growth and survival. ? 228: 367-381.
- Li, Z.L., Burritt, D.J. (2003). Changes in endogenous polyamines during the formation of somatic embryos from isogenic lines of Dactylisglomerata? with different regenerative capacities. Plant? Growth Regulation. 40: 65-74.
- Lingakumar, ,? Kulandaivelu,? G. (1998) .? Differential response of growth and photosynthesis in cyamopsistetragonoloba L. grown under ultraviolet-B and supplemental long wavelength radiations.? Photosynthetica. 35: 335- 343.
- Malik, A.U., Singh, Z. (2003). Abscission of mango fruitlets as influenced by biosynthesis of polyamines . Journal of? Horticulture? Science and Biotechnology. 78: 721-727.
- Mancinelli, A.L., Yang, C., Lindquist, P., Anderson, O.R., and Rabino, I. (1975). Photocontrol of anthocyanin synthesis. III. The action of streptomycin on the synthesis of chlorophyll and antocyanin. Plant Physiol. 55: 251-257.
- Martin- Tanguy, J. (2001). Metabolism and function of polyamines in plants. Plant Growth Regulation. 34: 135-148.
- Mirecki, R.M., and Teramura, A.H. (1984). Effects of ultraviolet-B irradiance on soybean. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion. Ind. J. Plant 74: 478-480.
- Stolari, R.S., Bloomfield, P. &Mcpeters, R.D. (1991). Total zone trends deduced from Nimbus 7 TOMS data. Geophys. Res. Lett. 18: 1015- 1018.
- Tang, w., Ronald, JN., Outhavong, V. (2004). Exogenously added polyamines recover browining tissues in to normal callus cultures and improve plant regeneration in pine. Physiology 122: 386- 395.
- Tiburico, A.F., Campos, J.L., Figueras, X., Besford, R.T. (1993). Recent advances in the understanding of polyamine functions during plant development. Plant Growth Regulation. 12: 331-34.
- Wang, W., Vinocur, B., Altman, A. (2003). Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. ? 218: 1-14.
- Wellburn, A.R. and Lichtenthaler, H. (1984). Formulae and program to determine total carotenoids and chlorophyll a and b of leaf extracts in different solvents. In: Advances in Photosynthesis Research (Sybesma, C. ed.,) MartinusNijhoff / Dr. W. Junk, The Hague. 2: 9-12.
[K.Bavithra, S. Muthulakshmi and K. Lingakumar. (2019); EFFECT OF SPERMIDINE (SPD) ON PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES IN ZEA MAYS L. UNDER UV-B STRESS. Int. J. of Adv. Res. 7 (Feb). 1092-1097] (ISSN 2320-5407). www.journalijar.com
Centre for Research and Postgraduate Studies in Botany, Ayya Nadar Janaki Ammal College (Autonomous ), Sivakasi, Tamil Nadu, India.