Vol. 7 (04) pp. 681-688 DOI: 10.21474/IJAR01/8871

MOLECULAR CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM BURNT SWEET CHEESE (CHHENAPODA).

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  • Research Scholar, P.G. Department of Zoology, Utkal University- 751004, Odisha, India.
  • Professor, P.G. Department of Zoology, Utkal University- 751004, Odisha, India.
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Abstract

The purpose of this study was to isolate and characterize lactic acid bacteria from fermented burnt sweet cheese (chhenapoda) by PCR based molecular methods for identification of the isolates, which may help to formulate the starter culture as well as in the biological preservation of foods. Both classical and PCR based molecular methods were used to identify the lactic acid bacteria isolates. A total number of 18 isolates have been recovered from chhenapoda and characterized with molecular tools. RAPD analysis was performed initially to cluster the isolates using two different primers 27F and 1492R. Species identification was based on sequence analysis of 16SrRNA gene. A single cluster of lactic acid bacteria PCR products was sequenced and subjected to BLAST. The isolate showed high similarity with Bacillus vallismortis based on nucleotide homology and phylogenetic analysis. Phylogenetic analysis was performed using software MEGA 7. The study concludes that Bacillus vallismortis can be used to formulate starter cultures for the commercial production of burnt sweet cheese (chhenapoda), in dairy industries.

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References

  1. Angmo, K., Kumari, A., Savitri and Bhalla, T.C. (2016): Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT- Food Science and Technology, 66:428-435
  2. Bozoudi, D., Kotzamanidis, C., Hatzikamari, M., Tzanetakis, N., Menexes,G. and Litopoulou- Tzanetaki,E. (2015): A comparison for acid production, proteolysis, autolysis and inhibitory properties of LAB from fresh and mature Feta PDO Greek cheese, made at three different mountainous areas. International Journal of Food Microbiology, 200:87-96
  3. Bujnakova, D., Strakova, E. and Kmet, V. (2014): In vitro evaluation of the safety and probiotic properties of Lactobacilli isolated from chicken and calves. Anaerobe, 29:118-127
  4. Cintas, L.M., Casaus, M.P., Herranz, C., Nes, I.F. and Hernandez, P.E. (2001): Review: Bacteriocins of Lactic Acid Bacteria. Food Science and Technology International, 7(4):281-305
  5. Federici, S., Ciarrocchi, F., Campana, R., Ciandrini, E., Blasi, G. and Baffone, W. (2014): Identification and functional traits of LAB isolated from Ciauscolo salami produced in central Italy. Meat Science, 98(4):575-58
  6. Felsenstein, J. (1985): Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39:783-789
  7. Fuller, R. (1989): Probiotics in man and animals. Journal of Applied Bacteriology, 66(5):365-378
  8. Giraffa, G. (2003): Functionality of enterococci in dairy products. International Journal of Food Microbiology, 88(2-3):215-222
  9. Junior, W.L.G. de Almeida, Ferrari, I.D.S., de Souza, J.V., da Silva, C.D.A., da Costa, M.M. and Dias, F.S. (2015): Characterization and evaluation of lactic acid bacteria isolated from goat milk. Food Control, 53:96-103
  10. Kargozari, M., Emam-Djomeh, Z., Gandomi, H., Partovi, R., Ghasemlou, M. and Martin, R. (2015): Identification of selected lactobacillus strains isolated from Siahmazgi cheese and study on their behaviour after inoculation in fermented sausage model medium. LWT- Food Science and Technology, 62(2):1177-1183
  11. Kebede, A., Viljoen, B., Gadaga, T., Narvhus, J. and Lourens- Hattingh, A. (2007): The effect of container type on the growth of yeast and lactic acid bacteria during production of Sethemi South African spontaneously fermented milk. Food Res. Int., 40:33-35
  12. Kimura, M. (1980): A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16:111-120
  13. Kumar, A. and Kumar, D. (2015): Characterization of Lactobacillus isolated from dairy samples for probiotic properties. Anaerobe, 33:117-123
  14. Kumar, S., Stecher, G. and Tamura, K. (2015) MEGA7: Molecular Evolutionary Genetic Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, (submitted)
  15. Liong, M.T. and Shah, N.P. (2005): Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol coprecipitation ability of Lactobacilli strains. International Dairy Journal, 15(4):391-398
  16. Lu, X., Yi, L., Dang, J., Dang, Y. and Liu, B. (2014): Purification of novel bacteriocin produced by Lactobacillus coryniformis MXJ32 for inhibiting bacterial foodborne pathogens including antibiotic resistant microorganisms. Food Control, 46:264-271
  17. Mercenier, A., Pavan, S. and Pot, B. (2003): Probiotics as biotherapeutic agents: Present knowledge and future prospects. Current Pharmaceutical Design, 9(2):175-191
  18. Ouwehand, A.C., Salminen, S., Isolauri, E. (2002): Probiotics: an overview of beneficial effects. Antonie Van Leewenhoek- Journal of Microbiology, 82(1-4):279-289
  19. Palomo, M., Gutierrez, A.M., Perez-Conde, M.C., Camara, C. and Madrid, Y. (2014): Se metallomics during lactic fermentation of Se-enriched yogurt. Food Chemistry, 164:371-379
  20. Reale, A., Di Renzo, T., Rossi, F. et al., (2015): Tolerance of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosusstrains to stress factors encountered in food processing and in the gastrointestinal tract. LWT- Food Science and Technology, 60(2):721-728
  21. Sambrook, J. and Russel, J. (2001): Molecular cloning. A laboratory Manual, Cold Spring Harbour Laboratory, New York
  22. Strompfova, V. and Laukova, A. (2014): Isolation and characterization of faecalBifidobacteria and lactobacilli isolated from dogs and primates. Anaerobe, 29:108-112
  23. Sullivan, L.O?, Ross, R.P. and Hill, C. (2002): Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality. Biochimie, 84(5-6):593-604
  24. Tabanelli, G., Montanari, C., Bargossi, E. et al., (2014): Control of tyramine and histamine accumulation by LAB using bacteriocin forming lactococci. International Journal of Food Microbiology, 190:14-23
  25. Todorov, S.D. and de Melo Franco, B.D.G., (2010): Lactobacillus plantarum: Characterization of the species and application in food production. Food Reviews International, 26(3):205-229
  26. Tserovska, L., Stefanova, S. and Yordanova, T. (2002): Identification of lactic acid bacteria isolated from Katyk, goat?s milk and cheese. Journal of Culture Collections, 3:48-52.

How to Cite This Article

Soumya Pragyan Das and Prafulla Kumar Mohanty. (2019); MOLECULAR CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM BURNT SWEET CHEESE (CHHENAPODA)., Int. J. of Adv. Res., 7 (04), 681-688, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/8871

Corresponding Author

Soumya pragyan das
P.G.Department of Zoology, Utkal University, Odisha-751004
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.