12Aug 2019

BACTERIAL CONTAMINATION OF SOME VEGETABLES SOLD IN MAJOR MARKETS IN ADOEKITI, NIGERIA.

  • Department of Medical Laboratory Science, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria.
  • Department of Medical Microbiology and Parasitology, College of Medicine, Ekiti State University, Ado-Ekiti, Ekiti State, Nigeria.
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Consumption of vegetables contaminated with pathogens is a common source of infections. This study investigated bacterial contamination of vegetables sold in major markets in Ado-Ekiti, Nigeria. Sixty samples comprising of 20 samples each of Brassica oleracea, Cochorus olitorius, and Amaranthus hybridus were examined. Bacteriological procedures were followed in the isolation and identification of bacteria from culture media. Antimicrobial susceptibility of the isolates was done using the disk diffusion method. Sixty six bacteria were isolated from 60 vegetable samples. Of these isolates, Salmonella spp recorded 43.3%, followed by Citrobacter freundii 18.3%, Klebsiella spp 15.0%, Enterobacter spp 11.7%, Proteus spp and Alcaligenes spp 5.0% each, Escherichia coli and Providencia spp 3.3% each and Vibrio spp 1.7%. The prevalence of the isolates on 20 samples of Brassica oleracea decreased in the order of Salmonella species 55.0%, Citrobacter species 20.0%, Alkaligenes species 10%, Pseudomonas aeruginosa, Enterococcus species, Escherichia coli, Proteus mirabilis and Providentia species 5% each. The most frequent bacteria isolated on 20 samples of Cochorus olitorius was Salmonella species 50.0%, followed by Citrobacter species 15.0%. Vibro species and Alkaligenes species recorded 5.0% each. Enterobacter species recorded the highest frequency 30.0% on the 20 samples of Amaranthus hybridus, followed by Salmonella species and Klebsiella species 25.0% each, Citrobacter species 20.0%, Pseudomonas aeruginosa and Escherichia coli 5.0% each. All isolates were sensitive to ofloxacin and ciprofloxacin but resistant to augmentin, ampicillin and cefuroxime. It is essential to control the sources of vegetable contamination to minimize risk of infections especially in vegetables consumed raw.

  1. Adabara, N.U., Ezugwu, B.U., Momojimoh, A., Madzu, A., Hashimu, Z., Damisa, D. (2012): The prevalence and antibiotic susceptibility pattern of Salmonella Typhi patients attending a military hospital in Minna Nigeria. Adv. Preventive Med. 2012: 1?4.
  2. CLSI document M100-S23 (M02-A11) (2003): Disc diffusion supplemental tables, Performance standards for antimicrobial susceptibility testing. The complete standard may be obtained from the Clinical and Laboratory Standards Institute, 940 West Valley Road, Suite 1400, Wayne, PA 19807
  3. Ajibade, V.A. (2012): Incidence of multidrug resistant; Salmonella sp in some food and meatproducts sold in Ado Ekiti, South Western Nigeria. Int J Sci Innos, 4 (1): 2276 ?6154.
  4. Avery, L.M.,?Killham, K.?and?Jones, D.L.?(2005):?Survival of?E. coli?O157: H7 in organic wastes destined for land application.?J Appl Microbiol., 98:814?822.
  5. Barber, L.B., Lee K.E., Swackhamer, D.L., Schoenfuss, H.L. (2007): Reproductive responses of male fathead minnows exposed to wastewater treatment plant effluent, effluent treated with XAD8 resin, and an environmentally relevant mixture of alkylphenol compounds. Aquat Toxicol., 82 (1):36?46.
  6. Barrow, G.I. and Feltam, R.k. (2004): Motility test: Cowan and Steels manual for the identification of medical Bacteria. 3rd Edition. Cambridge University Press: UK.
  7. Beuchat, L., and Ryu, J. (1997): Produce Handling and Processing Practices. Emerg Infect Dis,3(4): 459?465.
  8. Brackett, R.E. (1999): Incidence, Contributing Factors, and Control of Bacterial Pathogens in Produce. Postharvest Biology and Technology, 15(3): 305?311.
  9. Brandl, M.T.?(2006):?Fitness of enteropathogens on plants and implications for food safety.?Ann Rev Phytopath., 44:367?392.
  10. Chessbrough, M. (2006): District laboratory practice in Tropical countries 2. London: Cambridge University Press.
  11. Darwin, K., and Miller, V. (1999): Molecular Basis of the Interaction of with Salmonella the Intestinal Mucosa. Clin Microbiol Rev., 12 (3): 405?428
  12. David, O.M., Adedapo D.A., Moro, D.D., Esan C.O., Oje, O.J., Famurewa, O. (2015): Prevalence and carriage status of Salmonella typhi among students of Ekiti State University, Ado-Ekiti, Nigeria. Int J Biosci.,6 (10): 1-8.
  13. Eni, A., Oluwawemitan, I., and Solomon, O. (2010): Microbial Quality of Fruitsand Vegetables Sold in Sango Ota, Nigeria. Afr J FoodSci., 4(5):291?296
  14. Erkan, E.M. and Vural, A. (2008): Investigation of microbial quality of some leafy green vegetables. Food. Tech.,6: 285-288.
  15. Farooq, M., Anwar, F. and Rashid, U. (2008): Appraisal of heavy metal contents in different vegetables grown in the vicinity of an industrial area. Pak. J. Bot., 40:2099-2106.
  16. Gharavi, M.J, Jahani, M.R. and Rakni, M.B. (2002): Parasitic contamination of vegetables from farms and markets in Tehran. Iranian J. Publ. Health, 31: 83-86.
  17. Garrity, G.M., Brenner, D.I, Krieg, E. R., Stanley, J. T. (2005): Bergey?s Manual of Systemic Bacteriology, 2nd edition, volume 2. Springer-Verlag: New York.
  18. Hamilton, A., Stagnitti, F., Premier, R., Boland, A., and Hale, G. (2006): Quantitative Microbial Risk Assessment Models for Consumption of RawVegetables Irrigated with Reclaimed Water. Appl Environ Microbiol., 72(5): 3284?3290
  19. Heaton, J., Jones, K. (2008): Microbial Contamination of Fruit and Vegetables and the Behaviour of Enteropathogens in the Phyllosphere: A Review. J Appl Microbiol., 104 (3): 613?626
  20. Hung H. C., Joshipura K. J., Jiang R. H., Hunter D, Smith S. A. (2004): Fruits and Vegetable intake and the risk of major chronic disease. J Natl Cancer Inst., 95: 157- 164.
  21. Ibekwe, A. C., Okonko, I. O., Onunkwo, A. U., Donbraye, E., Babalola,E. T and Onoja, B.A (2008): Baseline Salmonella agglutinin titres in apparently healthy freshmen in Awka, South Eastern, Nigeria. Sci. Res. Essays, 3(9): 225-230.
  22. Itanna, F. (2002): Metals in leafy vegetables grown in Addis Ababa and toxicological implication. Ethiop. J. Health Dev., 16: 295-302.
  23. Machado, D., Maia, C., Carvalho, I., da Silva, N., Andre, M., and Serafini, A. (2006). Microbiological Quality of Organic Vegetables Produced in Soil Treated with Different Types of Manure and Mineral Fertilizer. Braz. J. Microbiol., 37(4): 538?544
  24. Natvig, E.E., Ingham, S.C., Ingham, B.H., Cooperband, L.R., and Roper, T.R. (2002): Salmonella enterica serovar Typhimurium and Escherichia coli contamination of root and leaf vegetables grown in soils with incorporated bovine manure. Appl Environ Microbiol., 68:2737?2744.
  25. Nguyen-the, C., and Carlin, F. (1994): The Microbiology of Minimally Processed Fresh Fruits and Vegetables. Crit Rev Food Sci Nutr., 34 (4): 371?401
  26. Olusegun, O. (2013). Urban expansion and urban land use in Ado Ekiti, Nigeria. American Journal of Research communication, 1 (2): 128-139.
  27. Oluyege, A. O., Babalola, J. A., Igbalajobi, A. O. and Oloruntuyi, A. B. (2015): Isolation and characterization of Salmonella typhi from Widal positive patients attending Ekiti State University Teaching Hospital. Int J. Curr. Microbol. App. Sci., 4 (10): 774-784.
  28. Oluyege, O. A. and Famurewa, O. (2015): Microbial Contamination and Antibiotic Resistance in Enteric Pathogens Isolated from Cooked Foods Sold in Eateries in Ado-Ekiti, Nigeria. British Microbiology Research Journal, 6(4): 236-246.
  29. Oranusi, S., Olorunfemi, O. J. (2011): Microbiological safety evaluation of street vended ready-to-eat fruits sold in Ota, Ogun state, Nigeria. Int J biol Sci., 1 (3): 22-26.
  30. Rosas, I., Baez, A., and Coutino, M. (1984): Bacteriological Quality of Crops Irrigated with Wastewater in the Xochimilco Plots, Mexico City, Mexico. Appl Envern Microbiol., 47 (5): 1074?1079.
  31. WPR [World Population Review] (2018). Population of cities in Nigeria. Accessed online at http://worldpopulationreview.com/countries/nigeria-population/cities/ 18/06/2019 5:22PM.
  32. Yusuf, I. Z., Oyaweye, O. M., Yongabi, K. A., Pemu A. T. (2004). Bacteriological Quality Assessment of Salad Vegetables sold in Bauchi Metropolis. Nigeria Journal of Microbiology. 18: 316-320.
 

[Oluwapelumi Bernard Oluboyo, Oluwabunmi G. Olojede, Funmilayo Janet Akinseye, Yomi Richard Akele, Adeola Olanrewaju Oluboyo And Funmilayo A. Adewumi. (2019); BACTERIAL CONTAMINATION OF SOME VEGETABLES SOLD IN MAJOR MARKETS IN ADOEKITI, NIGERIA. Int. J. of Adv. Res. 7 (Aug). 638-645] (ISSN 2320-5407). www.journalijar.com

Oluwapelumi Bernard Oluboyo
Department of Medical Laboratory Science, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria.

DOI:

Article DOI: 10.21474/IJAR01/9545      
DOI URL: https://dx.doi.org/10.21474/IJAR01/9545

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