15Nov 2019

A COMPARATIVE IN VIVO STUDY OF ANTIDIABETIC POTENTIALITY AND EVALUATION OF SAFETY PROFILE OF GYNURA PROCUMBENS, TERMINALIA CHEBULA AND FICUS RACEMOSA ON ALLOXAN - INDUCED DIABETIC RATS

  • Department of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, Bangladesh
  • BRAC University, 66 Mohakhali, Dhaka-1212, Bangladesh
  • Department of Pharmacy, East West University, Aftabnagar, Dhaka, Bangladesh
  • National Medical College, Dhaka, Bangladesh
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Leaves of Gynura procumbens (L.) (Family: Asteraceae) is one of the eminent leafy vegetable that is utilized for this purpose conventionally. It has been consumed against diabetes for long while. Diabetes was induced in rats by intraperitoneal injection of alloxan at a dose of 150mg/Kg bodyweight and ethanolic extract of leaves of Gynura procumbens was foraged to the rats at a dose of 750 mg/kg. We determined blood glucose level, and safety profile by determining SGOT, SGPT and creatinine on diabetic and non-diabetic rats before and after administration of the extract. After determining blood glucose level, it was detected that the hypoglycemic efficiency was analogous to that of metformin (p> 0.05) which was imparted at a dose of 500 mg/kg. Safety profile were probed by inspecting SGOT, SGPT and creatinine level. It was discerned that both metformin and leaf extract of Gynura procumbens recuperated the pathological state induced by diabetes. In addition to that, in healthy individual rats both metformin and leaf extract of Gynura procumbens did not substantially alter conventional physiological state. It can, hence, be conjectured that extract of leaves of Gynura procumbens could be exploited as a good alternative therapy to treat diabetes.

  1. Trivedi, B., Mazumdar, J.D., Bhatt and Hemavathi, K.G.(2004) Effect of Shilajit on blood glucose and lipid profilein alloxan-induced diabetic rats, Indian J Pharmacol. 36(6), 373-376.
  2. Rajasekaran, ,? Sivagnanam,? K.,? Narayanan,? V.? and Subramanian, S. (2001) Hypoglycemic and hypolipidemiceffects of Aloevera on experimental rabbits. Publicationof Indian Association of Biomedical Scientists. 41-45.
  3. Nawawi A, Nakamura N, Hattori M, Kurokawa M and Shiraki K: Inhibitory effects of Indonesian medicinal plants on the infection of herpes simplex virus Type 1. Phytotherapy Research 1999; 13: 37-41.
  4. Iskander M, Song Y and Jiratchariyakul WC: Anti-inflammatory screening of the medicinal plant Gynura procumbens. Plant Foods Hum Nutr 2002; 57: 233-244.
  5. Wong SK, Jann MLS, Sudi S, Hasan M, Chin LP, Embi N and Sidek HM: Anti-malarial and Anti-inflammatory Effects of Gynura procumbens are mediated by kaempferol via inhibition glycogen synthase kinase-3β (GSK3β). Sains Malaysiana 2015; 44(10): 1489-1500.
  6. Chan LK, Lim SY and Pan LP: Micropropagation of Gynura procumbens (Lour.) Merr. An important medicinal plant. Journal of Medicinal Plants Research 2009; 3: 105-111.
  7. Zhang XF and Tan BKH: Effects of an ethanolic extract of Gynura procumbens on serum glucose, cholesterol and triglyceride levels in normal and Streptozotocin-induced diabetic rats. Singapore Med J 2000; 41: 9-13.
  8. Akowuah GA, Sadikum A and Mariam A: Flavonoid identification and hypoglycaemic studies of the butanol fraction from Gynura procumbens. Pharm Biol 2002; 40: 405-410.
  9. Bohari M, Pauliena SH, Muhajir, Shaari, Lajis K and Nordin: Glucose uptake. Stimulatory activity of Gynura procumbens in 3T3- F442A adipocytes. Sarawak: Malaysian Medicinal Plant: Chemistry and Biological Activity: UNIMAS and Malaysian Natural Products Society, 2000.
  10. Rasadah MA, Hamid M, Saufi MB and Nik Musadah M: Study on anti-diabetic properties of Gynura procumbens Poster presented at Natural Products Seminar, Kota Kinabalu 2002; 23-24.
  11. Hassan Z, Yam MF, Ahmad M and Yusof AP: Anti-diabetic properties and mechanism of action of Gynura procumbens water extract in streptozotocin-induced diabetic rats. Molecules 2010; 15(12): 9008-23.
  12. Lam SK, Idris A, Bakar ZAA and Ismail R: Gynura procumbens and blood pressure in the rats: a preliminary study. Asia Pacific J of Pharmacology 1998; 13: 14-15.
  13. Hoe SZ, Kamaruddin MY and Lam SK: Inhibition of angiotensin-converting enzyme activity by a partially purified fraction of Gynura procumbens in spontaneously hypertensive rats. Med Princ Pract 2007; 16: 203-208.
  14. Hoe SZ, Lee CN, Mok SL, Kamaruddin MY and Lam SK: Gynura procumbens Decreases blood pressure in rats by vasodilatation via inhibition of calcium channels. Clinics, 2011; 66: 143-150.
  15. Balasuriya N and Rupasinghe HPV: Antihypertensive properties of flavonoid-rich apple peel extract. Food Chem 2012; 135: 2320-2325.
  16. Lokesh D and Shankar DA: Evaluation of a mechanism for antihypertensive action of Clerodendrum colebrookianum, used by folklore healers in north-east India. J Ethnopharmacol 2012; 143: 207-212.
  17. Borgohain R, Lahon K, Das S, Gohain K. Evaluation of Mechanism
  18. of Anti-diabetic activity of Terminalia Chebula on Alloxan and Adrenaline Induced Diabetic Albino Rats. Intern J Pharm and Bio Sci 2012;3(3):256-66.
  19. Bag A, Bhattacharyya SK, Pal NK, Chattopadhyay RR. In vitro antimicrobial potential of terminalia chebula fruit extracts against multidrug resistant uropathogens. Asian Pacific J Tropical Biomedicine 2012.p.1-5.
  20. Reddy DB, Reddy TC, Jyotsna G, Sharan S, Priya N, Lakshmipathi V, Reddanna P. Chebulagic acid, a COX-LOX dual inhibitor isolated from fruit of T. chebula Retz. induces apoptosis in COLO-20S cell line. J Ethnopharmacol 2009;124(3):506-12.
  21. Lee HS, Jung SH, Yun BS, Lee KW. Isolation of chebulic acid from Terminalia chebula Retz. and its antioxidant effect in isolated rat hepatocytes. Arch Toxicol 2007;81(3):211-8.
  22. Suchalatha S, Shyamala Devi CS. Protective effect of Terminalia chebula against experimental myocardial injury induced by isoproterenol. Indian J Exp Biol 2004;42(2):174-8.
  23. Tasduq SA, Singh K, Satti NK, Gupta DK, Suri KA, Johri RK. Terminalia chebula (fruit) prevents liver toxicity caused by sub-chronic administration ofrifampicin, isoniazid and pyrazinamide in combination. Hum Exp Toxicol 2006;25(3):111-8.
  24. Sharma P, Prakash T, Kotresha D, Ansari MA, Sahrm UR, Kumar B, Debnath J, Goli D. Antiulcerogenic activity of Terminalia chebula fruit in experimentally induced ulcer in rats. Pharm Biol 2011;49(3):262-8.]]]]]]]]
  25. Shiksharthi AR, Mittal S. Ficus racemosa: Phytochemistry, Traditional uses and pharmacological properties: a review. Int J Recent Adv Pharm Res 2011;4:6-15.
  26. Mandal SC, Maity TK, Das J, Saba BP, Pal M. Hepatoprotective activity of Ficus racemosa leaf extract on liver damage caused by carbon tetrachloride in rats. Phytother Res 1999;13:430-2.
  27. Mandal SC, Maity TK, Das J, Saba BP, Pal M. Antiiflammatory evaluation of Ficus racemosa leaf extract. J Ethnopharmacol 2000;72(1-2):87-92.
  28. Shaikh T, Rub R, Bhise K, Pimprikar RB, Sufiyan A. Antibacterial activity of Ficus racemosa Linn leaves on actinomycetes viscosus. J Pharm Sci Res 2010;2:41-4.
  29. Al-Amin, Md & Uddin, Mir Muhammad Nasir & Rizwan, Ashfique & Islam, Md. (2013). Effect of Ethanol Extract of Coccinia grandis Lin leaf on Glucose and Cholesterol Lowering Activity. British Journal of Pharmaceutical Research, ISSN: 2231-2919. 3. 1070-1078.
  30. Sharma, S.B.; Nasir, A.; Prabhu, K.M.; Murthy, P.S.; Dev, G. Hypoglycaemicand hypolipidemic effect of ethanolic extract of seeds Eugenia jambolana inalloxan-induced diabetic rats. Ethnopharmacol. 2003, 85, 201-206.
  31. Yin, Peipei & Wang, Yu & Yang, Lingguang & Sui, Jinling & Liu, Yujun. (2018). Hypoglycemic Effects in Alloxan-Induced Diabetic Rats of the Phenolic Extract from MongolianOak Cups Enriched in Ellagic Acid, Kaempferol and Their Derivatives. Molecules. 23. 1046.10.3390/molecules23051046.

[Zahirul Islam, Md. Naeem Hossain Rony, Farhana Farin, Amatun Noor Prapty, Md. Mehedi Hasan Khandoker, Sanjida Jannath, Tahmina Sharif Nila, Jannatul Nayeem and Fatema - Tuz - Zohra (2019); A COMPARATIVE IN VIVO STUDY OF ANTIDIABETIC POTENTIALITY AND EVALUATION OF SAFETY PROFILE OF GYNURA PROCUMBENS, TERMINALIA CHEBULA AND FICUS RACEMOSA ON ALLOXAN - INDUCED DIABETIC RATS Int. J. of Adv. Res. 7 (Nov). 604-610] (ISSN 2320-5407). www.journalijar.com

Zahirul Islam

DOI:

Article DOI: 10.21474/IJAR01/10049      
DOI URL: https://dx.doi.org/10.21474/IJAR01/10049

Download Full Paper

Download PDF No. of Downloads: 62 | No. of Views: 290