Identification and characterization of the trehalose gene (OtSA) in some pathogenic bacteria using an in silico approach

Trehalose-6-phosphate synthase gene (OtSA) plays an important role in pathogenic interactions between plants and microorganisms. The genomic database confirmed the induction of several genes of trehalose involved in pathogenesis. The analysis of the available bacterial genome sequence database in GenBank confirmed the presence of trehalose-6-phosphate synthase gene (OtSA) for trehalose biosynthesis in some non-pathogenic bacteria. E.coli, Pseudomonas and Salmonella genomes are known to have the OtSA gene sequence in their genomes with accession numbers FJ895834.1, AY308798.1 and NC003198, respectively. In the present investigation, the conserved regions among these genomes were used to construct the degenerate primers that were used to amplify a 530 bp of OtSA related sequences in these non-pathogenic bacteria . In addition ,we used the in silico approach to investigate the existence of a natural sequence of trehalose (OtSA) gene in some pathogenic bacteria .i.e. Pseudomonas aeruginosa (ATCC 9027) ,Bacillus cereus (ATCC 33018) , Pseudomonas luteola API-20 NE using the same pairs of the degenerate primers . The fragments of these three genes were amplified by PCR, then sequenced and submitted into the GenBank /EMBL/DDBJ. These three sequences were accepted with the accession numbers : KJ808665, KJ808666 and KJ808667 for Bacillus cereus, Pseudomonas luteola and Pseudomonas aeruginosa, respectively.