Polymorphic studies in PDX1/IPF1 Gene a key factor for the regulation of Insulin Gene

Diabetes is a major public issue due to its high prevalence and long-term complications. The molecular pathogenesis of diabetes, however, remains largely unknown. Diabetes is a result of a failure of the B cells of the endocrine pancreas to produce the amounts of insulin required to dispose of glucose, resulting in elevated blood glucose levels. Insulin is of vital importance in maintaining glucose homeostasis in mammals. This, and its unique role as the only anabolic peptide hormone, necessitates strict regulation and fast-acting mechanisms guaranteeing efficient insulin biosynthesis and secretion. In the present investigation primer Forward 5’-GCCGCAGACAATGGACTC-3’ and Reverse 5’-AGATGCCCTTGCTGCTGTCACC-3’ were used to detect the polymorphism among different diabetic blood samples. The amplification of the PDX 1 gene resulted in the amplification of the particular gene sequence in few Samples and the control sample and the PCR product was found to be around 320 base pair long after comparing with the marker. A blood sample showed no bands on the agarose gel which shows polymorphism in the particular gene in the given sample.