ISOLATION OF LIPASE PRODUCING BACTERIA FROM OIL AND FAT CONTAMINATED SOIL SAMPLES AND ASSESSMENT OF THEIR LIPOLYTIC EFFICIENCY TOWARDS DIFFERENT SUBSTRATES

  • Department of Biotechnology, Government Science College, Bengaluru, India.
  • Visveswarapura Institute of Pharmaceutical Sciences, Bengaluru, India.
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Lipases find immense applications in food, dairy, detergent and pharmaceutical industries as Biotechnologically significant enzymes. In this regard, an effort was made to isolate bacteria from oil and fat contaminated soil samples. Further, three pure bacterial cultures were characterized and their lipolytic efficiency towards different substrates was assessed. Bacillus spp. degraded olive oil effectively and sunflower oil was significantly degraded by Pseudomonas spp. Of the three bacteria, Staphylococcus spp. showed maximum lipolytic activity towards diesel followed by kerosene as substrates. Hence, we can infer that isolated Staphylococcus spp. can be used effectively in the bioremediation of fuel oil spills.

  1. Beisson, F., Tiss, A., Rivi?re, C. and Verger, R. (2000): Methods for lipase detection and assay: a critical review. Eur J Lipid Sci Technol., 133?153.
  2. Beller, H. R., Spormann, A. M., Sharma, P. K., Cole, J. R. and Reinhard, M. (1996): Isolation and characterization of a novel toluene-degrading, sulfate-reducing bacterium. Appl. Environ Microb., 62(4): 1188?1196.
  3. Bornscheuer, U.T. (2002): Microbial carboxyl esterases: classification, properties and application in biocatalysis. FEMS Microbiol Rev., 26 (1): 73?81.
  4. Creig, R. N. and Holt, G. J. (1984): Bergey?s Manual of Systematic Bacteriology. Williams and Willkins, London. Available from: https://www.worldcat.org/title/bergeys-manual-of-systematic-bacteriology/oclc/9042846.
  5. Gupta, J. K. and Soni, S.K. (2000): Industrial uses of enzymes. J Punjab Acad Sci., 2: 75?80.
  6. Ficker, M., Krastel, K., Orlicky, S. and Edwards, E. (1999): Molecular characterization of a toluene- degrading methanogenic consortium. Appl Environ Microbiol., 65(12): 5576- 5585.
  7. Gupta, J.K. and Soni, S. K. (2000): Industrial uses of enzymes. J Punjab Acad Sci., 2: 75?80.
  8. Jaeger, K.E., Ransac, S., Dijkstra, B. W., Colson, C., Heuvel M van. and Misset O. (1994): Bacterial lipases. FEMS Microbiol Rev., 15: 29?63.
  9. Jaeger, K.E. and Reetz, M.T. (1998): Microbial lipases form versatile tools for biotechnology. Trends Biotechnol., 16: 396?403.
  10. Jaeger, K.E., Dijkstra, B.W. and Reetz, M.T. (1999): Bacterial biocatalysts: molecular biology, three-dimensional structures and biotechnological applications of lipases. Annu Rev Microbiol., 53: 315? 351.
  11. Jensen, R. G. (1983): Detection and determination of lipase (acylglycerol hydrolase) activity from sources. Lipids., 18(9): 650-657.
  12. Joseph, B., Ramteke, P. W. and Thomas, G. (2008)?: Cold active microbial lipases?: Some hot issues and recent developments. Biotech Advances., 26: 457-470.
  13. Mobarak-Qamsari, E., Kasra-Kermanshahi, R. and Moosavi-nejad Z. (2011): Isolation and identification of a novel, lipase-producing bacterium, Pseudomnas aeruginosa KM110. Ran. J. Micro., 3(2): 92?98.
  14. Palekar, A. , Vasudevan, P.T. and Yan, S. (2000): Purification of lipase: a review.?Biocatalysis Biotransformation., 18: 177?200.
  15. Pandey, A., Benjamin, S., Soccol, C. R., Nigam, P., Krieger, N. and Soccol, U.T. (1999): The realm of microbial lipases in biotechnology. Biotechnol Appl Biochem., 29: 119?131.
  16. Ray, A. (2012): Application of lipase in industry. Asian J Pharm Technol., 2(2): 33?37.
  17. SaradaSarkar, B., Sreekanth, Shreya Kant., Rintu Banerjee and Bhattacharya, B.C. (1998): Production and optimization of microbial lipase. Bioprocess Engineering., 19: 29-32.
  18. Snellman, E. A. and Colwell, R. R. (2004): Acinetobacter lipases: molecular biology, biochemical properties and biotechnological potential. J Ind Microbiol Biot., 31(9): 391?400.
  19. Tolan, J. and Foody, B. (1999): Cellulase from submerged fermentation. Rec Progr Bioconv Ligno cellulosics., 41?67.

Rama T., Farissa D., Anil Kumar K.V. and Kiran Kumar D.J (2020); ISOLATION OF LIPASE PRODUCING BACTERIA FROM OIL AND FAT CONTAMINATED SOIL SAMPLES AND ASSESSMENT OF THEIR LIPOLYTIC EFFICIENCY TOWARDS DIFFERENT SUBSTRATES, Int. J. of Adv. Res., 8 (03), 869-874, ISSN 2320-5407. DOI URL: https://dx.doi.org/10.21474/IJAR01/10700

Rama T
Department of Biotechnology, Government Science College, Bengaluru, India

DOI:

Article DOI: 10.21474/IJAR01/10700      
DOI URL: https://dx.doi.org/10.21474/IJAR01/10700

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