Effect of non porine (49 K.D) outer membrane proteins of Salmonella typhimurium against Salmonellosis in mice leukocytes and phagocytosis

The research aimed to investigate the protective effect of non porins outer membrane proteins (OMPs) of Salmonella typhimurium against salmonellosis. The outer membrane non porin proteins (OMPs) of this bacterium was extracted and analyzed by Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The desired proteins Purification by ion exchange chromatography on DEAE- cellulose and gel filtration chromatography on Sephacryl S- 200 was carried out to obtain non porin proteins with Molecular weight 49 kDa. To evaluate the immunological parameters (total and absolute count of leucocytes, Phagocytic index) .For this purpose 15 albino mice divided into three groups before subjection to various treatments. The results of immunological parameters were as follows: Group III showed a significant increase in total count of leucocytes, neutrophils, monocyte and lymphocyte. No significant differences were detected in the total counts of. Group III showed a significant increase in phagocytic index and total immunoglobulin level.