Isolation and identification of Candida species from vaginal, urine and oral swabs by chromagar Candida

A total of 150 samples (50 vaginal swabs , 50 urine samples and 50 oral swabs) of patients at many age group range from 1to 50 year old and for both gender were collected from patients suffering from vaginal candidiasis, oral thrush and urinary tract infection who attending the Samawah Teaching Hospital for pediatrics and Gynecology of AL-Muthanna and AL-Diwanyia governorates ; Through the period which extended from October 2010 to May 2011. The isolation and identification methods of yeast isolates were followed upon the morphological, cultural and biochemical characteristics, in addition, the confirmative systems such as CHROMagar Candida and Api Candida were done for differentiation among Candida species. The phenotypic results showed that the isolation percent of Candida albicans was 63%, while the other species such as Candida glabrata, Candida krusei and Candida tropicalis were isolated with the ratios (14.2%, 8.69%, 7.6%) respectively. On the other hand, the percentage of yeasts such as Trichosporon sp. and the Geotrichum sp. were 5.43% and 1.08% respectively.