RECOMBINANT HETEROLOGOUS PROTEIN EXPRESSION IN BACTERIAL AND YEAST SYSTEMS

Recent studies have demonstrated that strain/genetic engineering is a very promising approach for evolving engineered E. coli strains with markedly enhanced capacities for recombinant protein production. Several unique and powerful methods have emerged recently that allow the generation of large libraries of bacterial and yeast mutants carrying different types of genetic profiles. Optimization of recombinant protein expression in prokaryotic and eukaryotic host systems has been carried out by varying simple parameters such as expression vectors, host strains, media composition, and growth temperature. The information obtained from the analysis of the genetic profiles in the isolated strains can provide invaluable and fundamental understanding about the biology of protein biogenesis, folding, stability and homeostasis in bacteria. This information can subsequently be combined and utilized to generate specialized protein expression in bacteria and yeast "cell factories" for uses in research as well as in the industrial field.