Effect of plant growth regulators and explant types on in vitro direct plant regeneration of Hyoscyamus reticulatus L.

Hyscyamus reticulatus L. is an important medicinal plant, belonging to Solanaceae family. This research was performed to study the effects of different explants (cotyledons, hypocotyls, shoot tips, and nodal segments) and various concentrations of 6-benzyladenine (BA) in combination with Indole-3-acetic acid (IAA) on shoot regeneration of Hyoscyamus reticulatus L. in vitro culture conditions. Regenerated shoots were transferred to half- or full-strength MS medium with various growth regulators for root regeneration. The experiment was done in completely randomized designs in four replications. Data was registered after nine weeks (three subcultures). Statistical analysis showed the highest average of regenerated shoots (162.25 shoot per treatment) was obtained on shoot tip explant in MS medium supplemented with BA (5mg.l-1) and IAA (0.1 mg.l-1) the lowest average of regenerated shoots (2.52, 1.75 shoot per treatment) respectively on cotyledon and hypocotyl explants in MS medium containing IAA (0.1 mg.l-1) and without BA and control treatment (0). Regenerated shoots have been rooted on full-strength MS medium containing 1.1 and 2.2 µM IBA and in other treatments no rooting induction was observed. Rooted plantlets derived from in vitro culture were transferred to plastic pots to acclimatization, with 90% survival. After 10 days they were transferred to greenhouse conditions.