PLASMID STABILITY STUDIESOF JM109 HOST SYSTEM

The purpose of the study delineates the growth and plasmid stability of E.coliJM109host system. Different concentrations of drugs, chemicals and various frequency of radiations were subjected to the host system to verify the colony forming units along with plasmid concentration and stability. Among chemicals, acridine orange showed highest effect on growth of DH5a, while among the drugs, danthrone showed maximum effect on the growth of the organism. Radio frequency of 2GHz and low intensity microwave radiation were recorded as highest inhibitory effects. However, there is no significant effect in growth was observed in exposure to UV rays. The present work discussed that, the effect of drugs, chemicals, radio frequency and microwave radiation have a huge effect not only on growth of organism but also concentration and stability of plasmid.