ARTIFICIAL FIELD INOCULATION OFBREAD WHEAT ADVANCED LINES WITH TILLETIAINDICA, CAUSAL AGENT OF KARNAL BUNT

The reaction to Tilletiaindica of one thousand and ninety twobread wheat advanced lines were evaluated in the field during the crop season 2016-2017. Sowing in beds with two rows was carried out on November 11 and 24, 2016, using 8 g of seed. Five spikes per line were inoculated by injection with 1 mL of an allantoidsporidial suspension (10,000/mL) during the boot stage, and at maturity the percentage of infection was determined by counting healthy and infected grains. The range of infection in the first date was 0-88.83 with a mean of 31.81%, while in the second date it was 0-82.65% with a mean of 24.44%.The range of infection of the two dates was 0.46-83.71% with a mean of 28.12%.Sixteenlines showed a percentage of infection equal or below 5.0% in both dates, and out of those lines, the following five showed less than 2.5%: two sister lines of MUNAL#1/FRANCOLIN#1*2/3/ATTILA*2/PBW65//MURGA(CMSS12Y00701T-099TOPM-099Y-099M-0SY-13M-0WGY), MUNAL#1/FRANCOLIN#1*2/3/ATTILA*2/PBW65//MURGA (CMSS12Y00701T-099TOPM-099Y-099M-0SY-17M-0WGY), BAJ#1/3/KIRITATI//ATTILA*2/PASTOR*2/4/MUTUS*2/TECUE#1, VILLAJUAREZF2009/6/ATTILA/3*BCN//BAV92/3/PASTOR/4/TACUPETOF2001*2/BRAMBLING/5/PAURAQ, and KACHU/BECARD//WBLL1*2/BRAMBLING/4/FRET2/TUKURU//FRET2/3/MUNAL#1. Lines with the highest percentage of infection were: BABAX/LR42//BABAX*2/3/KUKUNA/4/CROSBILL#1/5/BECARD/6/KSW/SAUAL//SAUAL/7/BABAX/LR42//BABAX*2/3/KUKUNA/4/CROSBILL#1/5/BECARD with 88.83 in the first date,MUU/KBIRD//2*KACHU/KIRITATIwith 84.77 and 82.65%in the first and second date, respectively, and TACUPETOF2001*2/BRAMBLING//WBLL1*2/BRAMBLING/6/WBLL1*2/KURUKU*2/5/REH/HARE//2*BCN/3/CROC_1/AE.SQUARROSA(213)//PGO/4/HUITES/7/BAV92//IRENA/KAUZ/3/HUITES/4/2*ROLF07 with 81.67% in the first date. The average of the three highest levels of infection of the susceptible checkwas99.7%.