Formulation and in-vitro evaluation of long circulating coated vitamin ETPGS phytosomes of docetaxel

Docetaxel (DCX) is a highly effective chemotherapeutic drug used in the treatment of different types of cancer, including non-small cell lung cancer (NSCLC). The drug is known to have low oral bioavailability due to its low aqueous solubility, poor membrane permeability and susceptibility to hepatic first-pass metabolism. To mitigate these problems, DCX is administered via the intravenous route. Coated vitamin ETPGS loaded phospholipid complex (phytosomes) was formulated by solvent evaporation method. .A new, simple, rapid , economical and sensitive UV - Spectrophotometric method has been developed for the estimation of docetaxel .The maximum wavelength of docetaxel was observed at 228 nm over the concentration range of 2 to 32μg/ml with R² = 0.9997. The formulations was characterized for particle size, zeta potential, entrapment efficiency, FTIR (Fourier transformed infrared), TEM (transmission electron microscopy) and drug release properties In-vitro drug release studies were carried out in phosphate buffer saline (pH 7.4) using dialysis bag method. The results indicates that the shape and size of the optimized (F2) formulation was confirmed through microscope and particle size and zeta potential found that most of the particles were well identified. After selecting the best formulation (F2) different concentrations (0.01 ,0.03, 0.05%) of vitamin ETPGS was added to the formulation (F5-F7) for coating of phytosome. The optimum formulation was selected further depending upon the particle size and entrapment efficiency of the prepared formulations and was found to be (F6) 86.6±0.0443%. The coated vitamin ETPGS formulation batch FD6 has particle size 166.23nm with PDI 305.3, zeta potential in range of 166.23nm with PDI 305.3. TEM & FTIR data show that the selected drug and excipients were chemically compatible. The results showed that the drug release of F6 formulation followed Higuchi order which describes that the docetaxel follows a sustain mechanism for release of coated vitamin ETPGS phytosome of docetaxel upto to72 hours. Thus, the results indicates that the formulation of coated vitamin ETPGS phytosomes of docetaxel could be developed as safe and beneficial.