Vol. 3 (07) pp. 30-34

High-molecular-weight DNA extraction from six Quercus species of Kumaun Himalaya, India

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  • G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora-263643 Uttarakhand, India.
  • Plant tissue culture and Molecular Biology Laboratory, Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital- 263136, Uttarakhand.
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Abstract

Quercus is an economically and ecologically important globally distributed genus of family fagaceae comprises of trees and shrubs. For genetic diversity, phylogenetic and evolutionary study of the species of this genus along their distribution, molecular tools can be beneficial. For them isolation of good quality DNA is prerequisite. High phenolic content of leaf tissue of Quercus makes it difficult to isolate good quality DNA free from phenol, lignin and pigments by conventional CTAB method. A reliable protocol for DNA isolation from frozen leaves of six Quercus species of Kumaun Himalaya has been standardized. The isolated DNA was found suitable for molecular studies and can be used to assess genetic diversity, phylogeny, and other molecular marker based studies.

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How to Cite This Article

Aseesh Pandey and Sushma Tamta (2015); High-molecular-weight DNA extraction from six Quercus species of Kumaun Himalaya, India, Int. J. of Adv. Res., 3 (07), 30-34, ISSN 2320-5407.

Corresponding Author

Aseesh Pandey

Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.