Exploring the microbiota of human milk using the culture-dependent method

Breast milk is vital sources of nutrients, also it consist of different antimicrobial compounds, immunoglobulin, immune component cells. Along with, it also contains a diverse microbial population which aid to initiation and development of infant gut microflora hence leads to stimulating growth and development of an infant. These diverse bacterial population also include the probiotic bacteria which stimulating a specific and nonspecific immunity and protect the neonate from intestinal disease, eczema, obesity and other infection due to the colonization of pathogenic bacteria. In the present study 31 milk samples were collected from healthy mother volunteers. Somatic Cell Counts of all samples perform and standardized in order to validate breast health. Based on phenotype and genotype characteristic, 27 bacteria were identified. The probiotic properties of all 27 isolates were checked out of which 12 isolates showed resistant to low pH and 11 isolates illustrated tolerance against 0.3% bile salt. In addition to this indicator organisms like Escherichia coli, Bacillus cereus, Bacillus subtilus, Pseudomonas aeruginosa, and Staphylococcus aureus were found to be most sensitive to 8 different isolates. The information generated from the present study reveals that breast milk is a resource of new life.