AFLP markers for the assessment of genetic variability in rose (Rosa gallica L.) cultivars in Tunisia
- National Gene Bank of Tunisia, Boulevard Leader Yasser Arafat Z. I. Charguia 1, 1080 Tunis, Tunisia.
- Department of Agricultural Production- Agricultural High School of Mograne, University of Carthage, 1121 Mograne, Tunisia.
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Genetic diversity of fourteen Ariana rose (Rosa gallica L.) cultivars collected from the north east of Tunisia was investigated using amplified fragment length polymorphism (AFLP). Three AFLP primer combinations produced a total of 533 polymorphic fragments with an average of 177.6 per primer combination. The percentage of polymorphic bands (89.8%), the resolving power (Rp; 99.7) and the PIC (0.51) values showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered; although similarity between cultivars was high (0.53-0.86) demonstrating a narrow genetic background. Cluster (UPGMA) and principal coordinate analyses (PCoA) indicate that the cultivars clustering made independently from the collecting origin. Our results showed that AFLP markers are useful for Rosa gallica L. germplasm discrimination as well as for investigation of genetic diversity and variation. The information will facilitate germplasm identification, conservation and new cultivar development.
[Najla Mezghani, Olfa Saddoud Debbabi, Slim Rouz and Maher Medini (2015); AFLP markers for the assessment of genetic variability in rose (Rosa gallica L.) cultivars in Tunisia Int. J. of Adv. Res. 3 (Jul). 35-42] (ISSN 2320-5407). www.journalijar.com
