Detection of gliotoxin in patients with pulmonary mycosis

Objective: This study was designed to detection of gliotoxin in patients with pulmonary Aspergillosis . Method: A total of 100 samples (sputum and blood) were selected from 300 outpatients who attended to Al-Qadissiyia Centre of Tuberculosis and Chest Diseases, , during the period from 2014 to May 2015. All patients had clinical manifestation in addition to 100 sample (sputum and blood) as control group. Results: all patients infected with fungi and three different genus isolated from sputum of patients ,these genus are Aspergillus sp 60 Isolates( 60%)with three species were A.fumigatus (50%)and A.flavus (33.3% )Cryptococcus neoformans (14%) Penicillum(8%) and Rhizopus sp(6%).So (71%) from patients had gliotoxin in their sputum and serum and (5%) had GT in their serum while (3%) of them had GT in their sputum. while 21 (21%) from patients didn't have GT in serum nor sputum. So this toxin was found in40(40%)in sputum and serum of control group , 6(6%)in sputum and 4(40%)in serum of control group serum the concentration of GT in sputum of patients was (40-63µg/kg) and in sputum of control group was (16-23µg/kg) and in serum of patients was (33-47µg/kg) and (10-21µg/kg) in serum of control group. and all A.fumigatus isolates 30 (100 %)were have gliz gene. Conclusion: several species of Aspergillus and other fubgi as well as possibly yeast, produced gliotoxin both in vitro and in vivo. More attention should be paid to this mycotoxin because of its multi-faceted toxic properties.