In vitro anthelmintic activity and phytochemical analysis of for tropical plants against Haemonchus contortus

The present study was conducted to test anthelmintic activity of various plants against Haemonchus contortus. The experimental study included the in vitro anthelmintic trials against adult parasites, larval stages and eggs of Haemonchus contortus. The adulticidal assay results revealed that adulticidal activity was maximum for Annona squamosa leaves MeOH (methanolic) extract (LC50 48.6 mg/ml at 1 hour post exposure) followed by Eucalyptus globulus leaves MeOH extract (LC50 50.81 mg/ml at 1 hour post exposure). The maximum larvicidal activity was shown by Catharanthus roseus leaves MeOH extract (LC50 11.30 mg/ml at 2 hour post exposure). Egg hatch assay revealed that the maximum ovicidal activity was attributed to Eucalyptus globulus leaves MeOH extract (LC50 7.06 mg/ml) and Annona squamosa leaves MeOH extract (LC50 5.8 mg/ml). Syzigium cumini was having minimum anthelmintic activity among selected plants. Anthelmintic activity of these plant extracts may be attributed to presence of alkaloids, tannins and essential oils in the crude extracts. The qualitative phytochemical analysis of the methnolic extracts of Eucalyptus globulus and Annona squamosa leaves showed the presence of these phytochemical constituents. TLC analysis showed the presence of various constituents at different Rf values.