Characterization and comparative analysis of Anabaena strain of rice fields of Manipur, India
Thingujam Indrama
, Keithellakpam Ojit Singh
, Gunapati Oinam
, Oinam Avijeet Singh
, Aribam Subhalaxmi
, Angom Thadoi
, Wangkhem Indira
, Chungkham Silvia
, Minerva Shamjetshabam
, Romi Khangembam
, Thiyam Bidyababy
, Kangjam Sarabati
, Onkar Nath Tiwari
and Gauri Dutt Sharma
- National Repository for Cyanobacteria and Microgreen algae (Freshwater), Microbial Resources Division,Institute of Bioresources and Sustainable Development (A National Institute of DBT, Govt. of India), Takyelpat, Imphal-795001, Manipur, INDIA.
- Department of Life Sciences and Bio-informatics, Assam University, Silchar-788011, Assam, INDIA.
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Thirty two different Anabaena strains were isolated from rice fields of Manipur, India, successfully cultured and characterized based on morphological and biochemical features under semi controlled conditions. Anabaena sp. BTA04 and BTA903 were investigated as high biomass in terms of chlorophyll-a producing and acetylene reduction activity expressing strains respectively. Anabaena sp. BTA03 was observed for the production of good content of total carotenoids and BTA35 for phycocyanin and allophycocyanin. Anabaena sp. BTA84 yielded high content of phycoerythrin in culture conditions. Biochemically potent Anabaena strains were deeply studied using amplified 16S rRNA gene analysis and compared with morphological characters. All strains were deposited to the National fresh water Cyanobacterial and Microalgal repository of DBT-IBSD, Imphal, Manipur with accession numbers. The sequences of the 16S rRNA gene of the five highly potent strains selected from this experiment have been submitted in the NCBI GenBank through GenBank submission tool Sequin software version 12.30 and got accession number as KM010233, KM010234, KJ562184, KF953534, KJ652540 for BTA03, BTA04, BTA35, BTA84, BTA903 respectively.
[Thingujam Indrama, Keithellakpam Ojit Singh, Gunapati Oinam, Oinam Avijeet Singh, Aribam Subhalaxmi, Angom Thadoi, Wangkhem Indira, Chungkham Silvia, Minerva Shamjetshabam, Romi Khangembam, Thiyam Bidyababy, Kangjam Sarabati, Onkar Nath Tiwari and Gauri Dutt Sharma (2015); Characterization and comparative analysis of Anabaena strain of rice fields of Manipur, India Int. J. of Adv. Res. 3 (Jun). 1-12] (ISSN 2320-5407). www.journalijar.com
