Molecular Study of CMV Isolated From Women with Repeated Miscarriage in Relation to Immune Response Molecule TLR2

This study was constructed to discuss a Molecular Study of Cytomegalovirus isolated from women with repeated miscarriage in relation to immune response molecule Tall like Receptor2. About (100) blood samples from women suffering from infection with Cytomegalovirus were collected from infertility clinic of Kamal Al –Sammaraee hospital and (50) samples from normal subjects served as control for comparison. Test subjects were divided into two age groups: 20-30 years old and 31-40 years old. The women distributed as (60) samples of infertile and (40) samples as miscarriage women, included molecular analysis by using polymerase chain reaction technique (PCR) for amplification two viral genes glycoprotein B (gB) that showed molecular weight 72bp and immediate early gene (IE2) that showed molecular weight 92bp. The amplification for TLR2 primer region showed molecular weight 419bp. After successful amplification of TLR2 gene good quality products were selected to be sequenced. The result showed that there is no mutation at TLR2 only replacement of C instead of G in wild type. PCR amplified for ILT2 primer region showed molecular weight of 285bp. Among ten Iraqi patients, only six give positive result when compared with healthy control; the type of mutation is deletion and substitution. The percentage of mutation types were deletion mutation (13.3%) and (86.7%) for substitution mutation. the effect of mutation was missense mutation (72.7%), Silent mutations (15.1%) and deletion mutations (12.2%).