Morphological and molecular characterization of potential cyanobacteria isolated from Loktak Lake
Ojit Singh Keithellakpam
, Indrama Thingujam
, Gunapati Oinam
, Avijeet Singh Oinam
, Minerva Shamjetshabam
, Silvia Chungkham
, Indira Wangkhem
, Bidyababy Thiyam
, Sarabati Kangjam
, Subhalaxmi Sharma Aribam
, Thadoi Angom
, Romi Khangembam
, Tiwari Onkar Nath
and Sharma Gauri Dutt
- Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (A National Institute of DBT, Govt. of India), Takyelpat, Imphal-795001, Manipur, INDIA.
- Department of Life Science and Bio-informatics, Hargobind Khurana School of Life Sciences, Assam University, Silchar-788011, Assam, INDIA
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In the present study, a total of ten (10) potential cyanobacteria isolated from Loktak Lake belonging to five (05) strains of Nostoc spp., three (03) strains of Phormidium spp., one (01) strain of Anabaena and one (01) strain of Calothrix sp. were investigated for their morphological characteristics and their phylogenetic relationship. These strains showed high phycobiliproteins content, extracellular ammonium excretion and nitrogenase activity when preliminary screening was conducted. A combination of morphological and 16S rRNA gene approach has been used for the first time to attest the phylogenetic affiliation of these potential strains. Intermingling of Nostoc and Anabaena species in 16S rRNA gene indicates that the two taxa are not clearly separated at the genetic level and may be polyphyletic in origin. In contrast to this, clustering of Calothrix and Nostoc species in the same clade attests the taxonomic coherence of these species. However, Phormidium showed greater phylogenetic diversity with all phylogenetic analyses. Distance matrix of 16S rRNA sequence showing similarities was ranged from 0.011-1.348. Despite a few minor incongruities, 16S rRNA gene depicted greater similarity with morphological taxonomy. Molecular characterization of cyanobacterial strains in the present study was to demonstrate their grouping and closeness of among them.
[Ojit Singh Keithellakpam, Indrama Thingujam, Gunapati Oinam, Avijeet Singh Oinam, Minerva Shamjetshabam, Silvia Chungkham, Indira Wangkhem, Bidyababy Thiyam, Sarabati Kangjam, Subhalaxmi Sharma Aribam, Thadoi Angom, Romi Khangembam, Tiwari Onkar Nath and Sharma Gauri Dutt (2015); Morphological and molecular characterization of potential cyanobacteria isolated from Loktak Lake Int. J. of Adv. Res. 3 (Jun). 19-27] (ISSN 2320-5407). www.journalijar.com
