Effect of Plant Growth Regulators on Indirect Organogenesis in Ruta graveolens.L
- KET’S V.G. Vaze College, Mithagar Road, Mulund (E), Mumbai, India
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Ruta graveolens is important medicinal and aromatic plant of Rutaceae. The present study was undertaken to evaluate the most suitable concentration of growth regulators for indirect organogenesis in Ruta graveolens.Callus was initiated from stem,and petiole on MS basal media supplemented with various combinations of auxins and cytokinines,the callus obtained from different explants was cultured on MS medium supplemented with various combinations of PGR’s.Studies were carried out to evaluate the shoot regeneration capacity of callus using plant growth regulators on growth of Ruta graveolens L. Among the different concentration of plant growth regulators used in the study;it was found that MS fortified with 4.44µM BA+2.69µM NAA resulted in highest shoot regeneration frequency 86.66% and highest mean number of shoots (28.00?1.15) with maximum shoot length (3.96 ? 0.3).
[Ajit Kengar, G.T. Paratkar (2015); Effect of Plant Growth Regulators on Indirect Organogenesis in Ruta graveolens.L Int. J. of Adv. Res. 3 (Jun). 1113-1119] (ISSN 2320-5407). www.journalijar.com
