Production, Purification and Characterization of Protease from Yersinia sp and Staphylococcus sp

Protease is one of the most important enzymes from industrial point of view. The production of protease was studied by submerged fermentation using Yersinia sp and Staphylococcus sp isolated from fish waste and slaughter house waste. The isolated strains were irradiated by UV rays to increase its enzyme production. Maximum protease production was achieved after 72 hours of incubation at pH 7.0 in 30°C for both parent and mutant strains of Yersinia sp and 48 hours of incubation at pH 8.0 in 30°C for both parent and mutant strains of Staphylococcus sp. Among the various carbon and Nitrogen sources were tested, lactose and skim milk are the best sources for maximum enzyme production by Yersinia sp., glucose and skim milk as the carbon and nitrogen sources for the production of protease by Staphylococcus sp.,. The molecular mass of the purified enzyme was determined by SDS-PAGE.