Interaction Effects of Different Nutrient Levels, Rhizobia and Arbuscular Mycorrhizal Fungi on Growth and Biomass of Dalbergia sissoo Roxb. seedling under Nursery Condition

A factorial randomized block experiment was conducted to determine the influence of different levels of Rhizobia and AMF under three levels of macronutrient (NPK) combinations on growth and biomass of Dalbergia sissoo seedlings. The treatment consist Nutrients (N1, N2, N3), Rhizobia (R1, R2, R3) and AMF (AMF1, AMF2, AMF3) alone and in its different combinations. The effect of different treatments and their combinations showed significant difference in all the attributes of plants compared to uninoculated control. In three way interaction, the maximum significant records for plant height, root length, shoot and root dry biomass were obtained in plants received with tripartite inoculation of N3xR3xAMF3 in which the magnitude of increase were 31.94, 32.5, 229.05 and 188.34% respectively compared to control, while N x AMF was found best among dual inoculation categories and nutrient added plants exhibited maximum growth and biomass among single treatment component. As for as the level of dose was concerned, the lowest level of nutrient, Rhizobia and AMF (N3xR3xAMF3) was scored Ist most effective treatment in biomass production (10.59 g pl-1) while the highest level of nutrient and lowest level of AMF (N1 x AMF3) was IInd best treatment (9.59 g pl-1 biomass). The moderate level of nutrient with lowest level of Rhizobia (N2x R3) scored IIIrd position (8.65 g pl-1 total dry biomass) compared to control (3.58 g pl-1 total dry biomass). Among treatments without any combination, the nutrient was found most effective as it increased the mean total biomass up to 62.01% while AMF inoculation registered 28.49% and Rhizobia 25.13% increase compared to control. It was also observed that there was synergistic interaction between Rhizobia and AMF, however this combination performed low than the treatment added with nutrient.