Application of hydrogel chitosan extract from the house fly (Musca domestica vicina) on burn wound healing of mice

Burn injury is first and foremost an injury to the skin. The burn wound healing process of mice skin was histologically described during the first seven and fourteen days. To evaluate the effects of chitosan on early extension of burn wound, deep partial-thickness burns were performed on the dorsum of mice. Mice were subjected to burn injury on their shaved dorsum, and they were subjected to topical hydrogel chitosan, euthanized and specimens of skin were sectioned and stained.The results showed marked increase in wound contractions areas of mice treated with 7 and 14 days with hydrogel chitosan. An increased re-epithelialization in chitosan-treated animals at day 14th of treatment, relative to the untreated burn control mice. The hydrogel-treated burns were so perfectly healed with intact re-grown epidermis, which was difficult to distinguish from normal skin. On day 7, a continuous incomplete epidermal layer was observed under the crust, and the number of inflammatory cells had decreased slightly. On day 14, a complete epidermal layer (comprising all the layers from the corneal to the basal layer). Also, rapid epithelialization was found in photomicrographs of wounds treated with the hydrogel indicated the wounds treated for 14 days shown improvement in wound healing activity compared to wounds treated for 14 days. These results indicate that chitosan induces could accelerate burn wound healing associated with diminished inflammation.