Molecular study to most important virulence factor of Entamoeba histolytica in Diwaniyah province
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The present study was conducted to investigate the infection of Entamoeba spp by using molecular technique (Quantitative real time polymerase chain reaction) of primers and probes for gene 18S rRNA . The results revealed the presence of Entamoeba histolytica in the percentage (74%) of 200 Entamoeba microscopic positive samples. Also three primers were used in this study for detection the gene of virulence factor of Entamoeba histolytica. The Cysteine protease revealed the high percentage(86.6%) of infection then Amebopore gene (80.35%) whereas Gal/lectin gene gave the lowest (7 5%) ,without significant differences at (p<0.05).
[Hiba Riyadth Jamil Al-Abodi , Hadi Madlul Hamzah Al-Mayali , Mansoor Jadaan Ali (2015); Molecular study to most important virulence factor of Entamoeba histolytica in Diwaniyah province Int. J. of Adv. Res. 3 (Aug). 231-236] (ISSN 2320-5407). www.journalijar.com
