Production, characterization and immobilised dye decolorization of amylase enzyme produced by Bacillus megaterium isolated from soil sample

Among different types of enzymes obtained from microbial sources, amylases are the most widely used in industries as well as on commercial basis. Amylase production from bacteria is economical as the enzyme production rate is higher in bacteria as compared to other microorganism. The present research work is focus on the production of amylase enzyme from Bacillus megaterium soil samples. Various fermentation conditions were checked for the production of amylase enzyme from isolated Bacillus megaterium, to improve the enzyme production. The optimum pH of Bacillus megaterium grows on pH 7. The optimum incubation time 48 hrs and temperature 37°C for Bacillus megaterium given the high yield of amylase enzyme. The extracted amylase enzyme was partially purified. Purification was performed by ammonium sulfate precipitation and DEAE-Cellulose column using ion exchange chromatography. The molecular mass purified amylase was estimated to be approximately 25 KDa by SDS-PAGE. The partially purified amylase enzyme was immobilised. The immobilized enzymes are used for removal of azo dyes by using different parameters. The maximum dye decolorization was found at an alkaline pH8.0 both the 5ml and 10ml enzyme containing 250 and 500 beads showed good adsorption rates.