EVALUATION OF CORNEAL WOUND HEALING ACTIVITY OF GLYCYRRHIZA GLABRA OPHTHALMIC FORMULATION BY IN-VITRO SCRATCH ASSAY
- Assistant Professor, Dept of Shalakya Tantra, PDEAs College of Ayurved and Research Centre, Nigdi, Pune, Maharashtra 411044.
- Professor and HOD, Dept. of Shalakya Tantra, Dr. D. Y. Patil College of Ayurved and Research Centre, Dr. D.Y. Patil Vidyapeeth (Deemed to be University ) Pune- 411018 Maharashtra, India.
- Professorand HOD, Dept. of Shalakya Tantra, Matoshri Ayurved College and Research Centre, Parner, Ahilyanagar,Maharashtra, India.
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Purpose:To formulate a novel ophthalmic formulation with Glycyrrhiza glabra for improving corneal wound healingbyin vitro scratch assay using Statens Seruminstitut Rabbit Corneal (SIRC) Cells.
Methods: In the present study, corneal wound healing activity was studied on statens serum institut rabbit corneal cell line. The method used to conduct the study was scratch assay method.The scratch was introduced into the confluent monolayer of cell line and treated with Glycyrrhizaglabra ophthalmic formulation. Evaluation of wound healing activity was compared with control group. Observations were noted at the beginning of study and at regular time intervals. Scratch measurement was observed. Quantitative parameters like percent wound closure, rate of cell migration, interlukin-6 concentration levels were measured. The study was performed according to protocols and standard operating procedures (SOP). All laboratory data has been accurately recorded and verified.
Result:The result of this experiment obtained with statistical analysis of trial group and control group. Results were expressed as mean+/-SD and data analysed by one-way, two-way analysis of variance (ANOVA) followed by turkeys multiple comparison test. From the results it can be observed that Glycyrrhizaglabra ophthalmic formulation has shown statistically significant increase in percent wound closure (p<0.05) after completion of treatment as compared to control wells. Which indicated itswound healing potential in SIRC cell line by scratch assay method.
[Sanika Abhay Virkar, Pallavi Jagtap and Mayur Shiralkar (2026); EVALUATION OF CORNEAL WOUND HEALING ACTIVITY OF GLYCYRRHIZA GLABRA OPHTHALMIC FORMULATION BY IN-VITRO SCRATCH ASSAY Int. J. of Adv. Res. (Jan). 274-283] (ISSN 2320-5407). www.journalijar.com
