ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF AYAPANA TRIPLINERVIS (VAHL) R.M. KING AND H.ROB

Ayapana triplinervis (Vahl) R.M.King and H. Rob is a tropical herb which belongs to the family Asteraceae. The present investigation is focused on the analysis of phytochemical constituents, antioxidant and antimicrobial activities of the medicinal plant, A.triplinervis. In the present study methanol extract of leaves of A.triplinervis was subjected to preliminary phytochemical screening. Here the plant showed the presence of carbohydrate, proteins, phenols, flavonoids, tannins and oils. The total phenol and flavonoid contents were also analysed. The results showed that the fresh leaves of A. triplinervis had good levels of flavonoids and phenol. Antioxidant activity of the methanol extract was determined using DPPH radical scavenging assay and reducing power assay. The antioxidant activity increases as the concentration of extract increases. The plant extract showed antibacterial activity against Bacillus subtilis and Vibrio cholerae.