PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF ESBL-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA SPP. IN A TERTIARY CARE TEACHING HOSPITAL IN SOUTH INDIA
- Senior Resident, Department of Microbiology, Sree Mookambika Institute of Medical Sciences, Kulasekaram, Kanyakumari, Tamil Nadu, India.
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Background: Extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae represent a serious challenge to antimicrobial therapy globally. This study assessed the prevalence of ESBL producing Escherichia coli and Klebsiella spp., their antibiotic resistance profile, and the distribution of ESBL genes. Methods: A cross-sectional study was conducted from July 2015 to June 2016 at a tertiary care hospital in Tamil Nadu, India. A total of 100 clinical isolates (50 E. coli and 50 Klebsiella spp.) from various specimens were identified using standard microbiological techniques. ESBL screening was done using third-generation cephalosporins, and confirmation employed the double-disk synergy test (CLSI 2010). PCR was performed to detect bla\TEM, bla\SHV, and bla\CTX-M genes.
Jasmine Gnana Sutha (2026); PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF ESBL-PRODUCING ESCHERICHIA COLI AND KLEBSIELLA SPP. IN A TERTIARY CARE TEACHING HOSPITAL IN SOUTH INDIA, Int. J. of Adv. Res., 14 (05), 1415-1418, ISSN 2320-5407. DOI URL: https://dx.doi.org/10.21474/IJAR01/23545
Senior Resident, Department of Microbiology, Sree Mookambika Institute of Medical Sciences, Kulasekaram, Kanyakumari, Tamil Nadu, India.
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