CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF UNKNOWN BACTERIA ISOLATED FROM OUTLET OF ARAB EL MADABEGH SEWAGETREATMENT PLANT IN ASSIUT CITY, EGYPT.
- Environmental Researcher at the Egyptian Environmental Affairs Agency (EEAA) Email: From_Assiut@hotmail.com.
- Environmental Researcher at the Egyptian Environmental Affairs Agency (EEAA) Email: che_mma85@yahoo.com.
- The holding company for Drinking and wastewater in Assiut, Egypt Email: mostafa_almalwany@yahoo.com.
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The aim of this study isolation, characterization and molecular identification of unknown bacteriafrom the outlet of sewage treatment plant (Arab El Madabegh station) on the Nile River inthe Assiut governorate, Egypt. Bacterial strain was isolated and characterized using various biochemical tests and confirmed through molecular approach. Bacterial 16S rRNA gene was amplified using suitable primers. The amplified 16S rRNAgene sequence was compared with the sequence in NCBI sequence database. The bacterial strain was identified as Salmonella .sp m33 (NCBI Gene Bank Accession No: KR027920.1) Phylogenetic and molecular evolutionary analyses were conducted using 16SrRNA sequencing. Salmonella .sp is a gram negative, rode shaped bacteria which forms white raised irregular colony. The sequence when submitted to NCBI gene bank database using BLAST showed 95– 97% maximum identity and E-value equal to 0 for all closely related taxa.
[Momen Salah Kamel Mahmoud, Mahmoud Mohamed Abdelshafy, Mostafa Abdelshafy Ramadan (2015); CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF UNKNOWN BACTERIA ISOLATED FROM OUTLET OF ARAB EL MADABEGH SEWAGETREATMENT PLANT IN ASSIUT CITY, EGYPT. Int. J. of Adv. Res. 3 (Oct). 182-187] (ISSN 2320-5407). www.journalijar.com
