Chemical Composition and biological Activity of aqueous and methanolic extracts of Thyme (Thymus vulgaris)

This study aimed to investigate the possible biological activity of aqueous, methanolic and sulfated extracts of tow medicinal plants thyme (Thymus vulgaris) and Ginger (Zingiber officinale) at 85 °C under acidic, neutral and alkaline conditions. Fibrinolytic, anticoagulation, antioxidant, antitumor and antimicrobial activities were investigated. The Biochemical studies of the chosen two fungi showed the presence of varying amounts and different forms of total ash, protein , lipid, and total carbohydrates. However, TLC chromatography examination showed that the output of the acid hydrolysis to the plants varying from monosaccharaides. Anticoagulation and fibrinolytic properties of the crude extracts as well as of their sulfated derivatives, at 2 mg/ml of extract were tested and show that the sulfated acidic, sulfated neutral and sulfated alkaline extract of T. vulagaris and sulfated neutral, alkaline extract , sulfated alkaline and methanolic extract of Z. officinale exhibited fibrinolytic activities equivalent to double of the same amount of standard "Hemoclar" . On the other hand, results also showed a promising anticoagulant activities of the sulphated extracts at different concentrations compared with the corresponding native extracts. Also, their antimicrobial activity of the aqueous extracts against both tested Gram positive and negative bacteria, yeast and fungal strains was determined. The chemical modification of the aqueous sulfation can increase significantly its anticoagulation and fibrinolytic activities. And also their extracts show antioxidant activity reached up to 68% comparative by standard. However, the antitumor activity is high for both the native and the sulfated aqueous extracts.