Histophathological, histochemical and biochemical studies on rat\'s liver as biomarkers for environmental pollution with insecticide

The present study was planned to evaluate the histopathological and histochemical effects of organophosphorus Chlorpyrifos insecticide on serum and liver of the white rat Rattu, norvergicus. The rats classified into two major groups ,first group was received 1/10 of LD50 for 0,2,7,10,14,21 days, of Chlorpyrifos ,the second group was administered with 1/2 of LD50 for 0,2,4,6 days ,and then left for 15 days for recovery Serum levels of AST,ALT,ALP were significantly increase with both 1/10 and 1/2 LD 50. Histopathological investigation showed that hepatic tissues exhibited dilated and congested portal vessels with perivascular mild lymphocyte infiltration in cases of single and repeated doses .Also, development of focal necrosis in the liver . The histochemical examination indicated depletion of the polysaccharides and protein content of the liver. This study showed that Chlorpyrifos caused increased liver enzymes with histopathological and histochemical effects in liver .