Matrix solid phase dispersion extraction for Tropane alkaloid detection in cultured and collected Hyoscyamus reticulatus plants
- PhD student. Iran, Tehran, Science and Research Branch, Islamic Azad University, Department of Horticultural Science.
- Lorestan University of Medical Sciences, Iran.
- Lorestan University • Department of Chemistry, Iran.
- National Institute of Genetic Engineering and Biotechnology (NIGEB), Iran.
- Department of Tissue culture and Gene Transformation Agricultural Biotechnology Research Institute of Iran (ABRII)
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Tropane alkaloids are important secondary metabolites with many medicinal uses. Solanaceae family and Hyoscyamus genus is an important source for this natural products. Hyoscyamine and scopolamine are important compounds that belong to tropane alkaloids class. For evaluation of scopolamine and hyoscyamine concentration, cultured and collected plants were analyzed by HPLC. For plant material extraction MSPD method was used. Results showed varied hyoscyamine and scopolamine concentrations in different organs. Maximum tropane alkaloid concentration found in the leaf of both cultured and collected plants. In cultured plant’s leaf, hyoscyamine was more than 6.5 times as much as collected plants and this rate for scopolamine was 7.6.
[Bardia Moradi, Bahram Delfan, Peyman Hashemi, Amir Mousavi, Hassan Rahnama (2015); Matrix solid phase dispersion extraction for Tropane alkaloid detection in cultured and collected Hyoscyamus reticulatus plants Int. J. of Adv. Res. 3 (Nov). 78- 82] (ISSN 2320-5407). www.journalijar.com
