ISOLATION, BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF CELLULASE PRODUCING FUNGI
- Department of Biotechnology, Arni University, Kathgrah (H.P) India.
- Department of Biotechnology, Lovely Professional University, Jalandhar, India.
- Department of Civil Engineering, Arni University, Kathgrah (H.P) India.
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Cellulose is the most abundant organic polymer which may be hydrolyzed by enzyme cellulase into smaller sugar components including glucose subunits which can be used for production of ethanol, organic acids and other chemicals. Five different isolates were isolated from collected samples and one was shown maximum zone of hydrolysis which was identified as Aspegillus sp. based on morphological, microscopic and molecular level. The best conditions like pH, carbon source, nitrogen source, and temperature and incubation period were also observed for cellulase producing fungi. Incorporation of fructose as carbon source, ammonium sulphate as nitrogen source and pH 6.0 gave best enzyme production by Aspergillus sp. The highest enzyme activity was observed on 6th day of incubation at temperature 28ºC. The unknown isolates were identified by amplifying and sequencing of 18S rRNA gene. After amplification the length of amplified DNA was obtained as 532bp and amplified PCR product was compared with sequence obtained from NCBI. A Phylogenetic tree was formed on the bases of comparison.
[Navita Pathania, UC Pachouri, Arjun Kumar (2015); ISOLATION, BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF CELLULASE PRODUCING FUNGI Int. J. of Adv. Res. 3 (Oct). 842-858] (ISSN 2320-5407). www.journalijar.com
