Iraqi Genotyping of Giardia lambila (A,B,E,F) in Human Stool In AL-Muthanna Province –Iraq

This study was carried out in AL-Muthanna Province during the period from October 2014 to March 2015 for detect the prevalence of G. lambila and determining genotypes of this parasite(A,B,E,F,). A total of 200 stool samples (male and female), were taken from human aged (>1-40>) years who suffering from acute or persistent diarrhea and it examined by flotation methods and Conventional-PCR assay, that the results showed total infection percentage with Giardia lamblia was (25%).Also the present study showed the total number of Giardia lamblia genotypes (A,B,E,F) for positive samples from PCR was 73 samples that the highest infection of assemblage A was 30.14% more than assemblage B was 26.03% and assemblage E and assemblage F in percentage ratio 20.55% and 23.29% respectively, without significant differences at level (P>0.05). The results showed that the PCR product of triosephosphate isomerase (TPI) gene that using in detection Giardia lamblia genotype (Assemblage A) that the positive samples isolates at 576bp PCR product size and the results that the PCR product of triosephosphate isomerase (TPI) gene that using in detection Giardia lamblia genotype (Assemblage B) that the positive samples isolates at 208bp PCR product size. While the results the PCR product of triosephosphate isomerase (TPI) gene that using in detection Giardia lamblia genotype (Assemblage E) that the positive samples isolates at 416bp PCR product size and the results of the PCR product of triosephosphate isomerase (TPI) gene that using in detection Giardia lamblia genotype (Assemblage F) that the positive samples isolates at 323bp PCR product size. The results of current study showed non significant differences at level ( P>0.05) between all age groups depended on molecular assay, also the results showed the highest percentage of infection in age group (>1-10) years in old was (60%) while the lower percentage of infection in both age groups (21-30) years and (31-40>) was (4%). The present study showed non significant differences at level ( P>0.05) between male and female depending on molecular assay, also the results showed the highest percentage in female was (52%) while the lower percentage in male was(48%). The present study showed that percentage of Giardia lamblia infection in rural regions was (66%) which is higher than urban regions (34%) with significant differences at level (P<0.05).The results of the current study showed that the highest rate of infection with Giardia Lamblia depended on molecular assay was in November and January in percentage ratio (30%) while the lower rate was in March was )0.00%).