ANTI-BACTERIAL ACTIVITY OF ZINC OXIDE NANOPARTICLES PREPARED FROM BRASSICA OLERACEAE LEAVES EXTRACT

Present study focuses on the green synthesis of ZnO nanoparticles by zinc nitrate and utilization of bio components of leaves extract of Brassica oleraceae. The characterization of the nanoparticles was examined by UV- Vis spectrum and scanning electron microscope (SEM). We investigated the antibacterial activity of ZnO nanoparticles with various particle size (1-100nm). on gram positive and gram negative bacterial strains by agar well diffusion method and their MIC and MBC values were determined. Antibacterial activities against E. coli, V. cholera, C. Botulinum, S.aureus, B. subtilis were evaluated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results indicate that zinc oxide nanoparticles had strong antibacterial activity against bacteria. The activity increased as the concentration of the nanoparticles increased. The MIC and MBC were 50ug/ml and 25?g/ml, respectively. Our study indicates that zinc oxide nanoparticles could potentially be an antibacterial reagent to treat diseases caused by these bacteria.