Generation of avian influenza (H5N1) virus-like particles from an Egyptian isolate using baculovirus expression system

Influenza virus-like particle (VLP) vaccines are one of the most promising approaches to respond to the emerging threat of pandemic strains, as they possess the potential for higher production capabilities compared to traditional influenza vaccines made in egg-based technology. Highly pathogenic avian influenza (HPAI) H5N1 virus became endemic in Egypt due to defect in the application of AI control strategies and shortage in the vaccine doses. Hence, our study was focused on generation of influenza H5N1 VLP from Egyptian isolate A/Turkey/Egypt/7/2007 (H5N1), using insect cell culture and baculovirus expression system. The generated influenza VLPs were observed photographed by electron microscope and detected by measuring hemagglutination activity, hemadsorbtion activity, SDS-PAGE, and Western plot. This study discuss the creation challenge of identified Egyptian isolate influenza VLPs vaccine production and processing in Baculovirus expression and cell system which could be in large scale is more rapid and easy than other production technologies. It is essential that further immune response results on the Egyptian isolate generated Influenza (VLPs) carried out which is in processing.