Development of Microsatellite (SSRs) markers and Evaluation of Genetic variability within Sugarcane commercial varieties (Saccharum spp. hybrids)
- Department of Biotechnology, Punjab Technical University (PTU), Jalandhar-144601, (Punjab), India.
- Swami Satyanand College of Management and Technology, Amritsar-143001, (Punjab), India.
- Sugarcane Research Institute, U.P. Council of Sugarcane Research, Shahjahanpur-242001 (U.P.), India.
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Sugarcane microsatellites markers were developed from ESTs and utilized for evaluation of genetic diversity of elite sub-tropical Indian commercial cultivars. Publically available EST database (dbEST/GenBank) offers a valuable resource for developing EST-SSRs markers. ESTs sequences, related to sucrose metabolism and yield traits were harvested from NCBI database and used to microsatellites (SSRs) identification by online SSR search tools. Among the identified SSRs types, tri-nucleotide repeats were frequently abundant (41.0%) followed by tetra-nucleotide repeats (22.4%), di-nucleotide repeats (19.1%), penta-nucleotide repeats (12.0%) and hexa- tri-nucleotide repeats (5.5%). Among 48 primer pairs, 29 (60.0%) were found to be polymorphic, 11(23.0%) monomorphic and 8 (17.0%) un-amplified at any temperature (Ta). Polymorphic markers were used to test the genetic diversity within a panel of 26 Indian sub-tropical sugarcane elite varieties. Most of the EST-SSRs markers were found to be informative and effective to revealing genetic variability within the sugarcane varieties of diverse pedigree. Thus, the developed EST-SSRs markers would be used as potential molecular tools in genetic mapping, phylogenetic analysis and in marker assisted selection (MAS) strategies in sugarcane.
[Ram Baran Singh, Balwant Singh and Ram Kushal Singh (2015); Development of Microsatellite (SSRs) markers and Evaluation of Genetic variability within Sugarcane commercial varieties (Saccharum spp. hybrids) Int. J. of Adv. Res. 3 (Dec). 700-708] (ISSN 2320-5407). www.journalijar.com
