Endotoxin exposure alters viability and apoptotic potential of buffalo neutrophils
- Upkardeep Singh: PhD Scholar, Canadian Centre for Health and Safety in Agriculture, University of Saskatchewan, Saskatoon, Canada.
- Professor, School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab and Adjunct Faculty, Department of Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada; email: email@example.com.
- Assistant Scientist, School of Animal Biotechnology, GADVASU, Ludhiana, Punjab, India 141004.
- Assistant Biochemist, School of Animal Biotechnology, GADVASU, Ludhiana, Punjab, India 141004.
- Assistant Scientist School of Animal Biotechnology, GADVASU, Ludhiana, Punjab, India 141004.
- Professor cum Director, School of Animal Biotechnology, GADVASU, Ludhiana, Punjab, India 141004.
- Cite This Article as
- Corresponding Author
Buffalo which contributes towards the social well being of millions of South East Asian farmers commonly suffer from various infectious diseases. There is paucity of literature on the innate immune response of buffalo specially the cells like neutrophils which are the first line of defense. Buffalo neutrophils were isolated by using Hi Sep LSM-1077 along with Dextran and viability was determined by trypan blue exclusion test. Presence of myeloperoxidase suggested presence of neutrophils. The freshly isolated neutrophils showed a purity of 98.4% with viability up to 98.44 ± 0.35%. There was a significant decrease (p<0.05) in the number of viable neutrophils after 24 hr, 48 hr and 72 hr post-incubation in the control group. LPS challenge showed significantly (p<0.05) more number of viable neutrophils at 48 and 72 hr compared to non LPS group. Buffalo neutrophils undergoing apoptosis showed condensation of nucleus and presence of apoptotic bodies with intact cell membrane. LPS challenge resulted a significant decrease in the number of apoptotic neutrophils compared to control group at different time intervals. It is the first data on the viability and apoptotic potential of neutrophils of water buffalo suggesting that LPS treatment activated the neutrophils which led to significant reduction in apoptosis and increased life span of neutrophils as compared to untreated cells.
[Upkardeep Singh, R S Sethi, CS Mukhopadhyay, BV Sunil Kumar, Dipak Deka and Ramneek Verma (2015); Endotoxin exposure alters viability and apoptotic potential of buffalo neutrophils Int. J. of Adv. Res. 3 (Dec). 1636-1644] (ISSN 2320-5407). www.journalijar.com
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