Effect of Ethanole and Methanole Extraction of Mentha piperitaL. leaves on some Pathogenic Bacteria , Cellular Immune Response and Phagocytosis in Rabbits

This experiment was conducted to evaluate the effect of ethanol and methanol extraction of (Mentha piperita) on some pathogenic bacteria , cellular immune responses and phagocytosis in rabbits . Eight pathogenic bacteria used in this study. Antimicrobial activity of M. piperita was evaluated by disk diffusion methods. The inhibition zone was found ranging from 10.00 to 18mm in ethanolic extraction. The inhibition zone was found ranging from 10.00 to 24mm in methanolic extraction and the highest zone of inhibition produced ethanol and methanolic extract against salmonella species .Twelve rabbits were used , and the cellular immune response done by Skin test about (1ml) of soluble antigen of salmonella spp. was injected in the of groups , this experiment showed that group ( A , B) : after 24 hours show , redness area and thickness of skin at sit of injection . The thickness measured by vernier caliper after 24 hours about (2.10±0.01mm), (2.95±0.130 mm) respectively , and the thickness of group ( A , B ) after 48 hours (1.70±0.06 mm) , (1.87±0.1.40 mm ) respectively . The phagocytosis in presented Mentha piperita extraction decreased the count of bacteria in group A(with ethanol extraction) about 86.75% and reached to 25.75% , in group B (with methanol extraction) about 73.25% and reach to 18.5% .